1,1,1,3,3,3-hexafluoropropan-2-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Nov - 26 Dec 1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1,3,3,3-hexafluoropropan-2-ol

EC Number:

213-059-4

EC Name:

1,1,1,3,3,3-hexafluoropropan-2-ol

Cas Number:

920-66-1

Molecular formula:

C3H2F6O

IUPAC Name:

1,1,1,3,3,3-hexafluoropropan-2-ol

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: location: 10 sites (4 sewage plants, 3 rivers, 1 lake and 2 bays) around Japan
- Temperature: 25 ± 1 °C
- Concentration of sludge: 5800 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

DOC removal

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: recommended medium according to the guideline
- Test temperature: 25 ± 1 °C
- Suspended solids concentration: 30 mg/L

TEST SYSTEM
- Culturing apparatus: open oxgen consumption measuring machine with 300 mL culture bottle
- Number of culture flasks/concentration: 1 (water + test substance), 3 (sludge + test sub.), 1 (sludge + reference sub.), 1 (sludge)
- Measuring equipment: Coulometer

SAMPLING
- Sampling frequency: Day 7, 14, 21 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: 1

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

Due to the volatility of the test item, an open or closed system was preliminarily tested. The residual substance was analysed by GC after 28 days.
(Water + test item) with soda lime in the open system: 84 and 87%
(Water + test item) without soda lime in the open system: 56 and 48%
(Water + test item) with soda lime in the closed system: 97%
(Water + test item) without soda lime in the closed system: -

In conclusion, TOC and GC was performed to analyze the test item in the open system without soda lime.

% Degradation

% Degradation 1

Parameter:

% degradation (TOC removal)

Value:

0

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Value:

5

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The oxygen consumption for aniline in the above used test system reached 73% and 72% degradation after 7 days and 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed