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EC number: 265-016-4 | CAS number: 64683-40-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Oxidation reduction potential
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- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
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- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995
- GLP compliance:
- no
- Vehicle:
- no
- Details on test solutions:
- Taking into account the value of 90% of active material, the tests will be carried out with the 100% active material of the test material.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Urban Water Treatment Plant in Manresa (Spain)
- Preparation of inoculum for exposure: The dry weight of the activated sludge from the urban sewage treatment plant (7.2 g /L) is calculated and suspended in water to a concentration of 4 g/L of suspended matter, so that when added 200 ml of these suspended sludge yields a final concentration of 1.6 g / L.
- Initial biomass concentration: Concentration of activated sludge (solids in suspension per liter): 1.6 g / L - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 ºC
- pH:
- 7-8
- Dissolved oxygen:
- see tables
- Nominal and measured concentrations:
- 10 mg/L, 32 mg/L, 100 mg/L, 320 mg/L and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Material, size, headspace, fill volume: 500 mL
- Aeration: continuos, approximate flow of 1 liter / minute
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 1.6 g/L
- Nutrients provided for bacteria: synthetic residual water
- Nitrification inhibitor used (delete if not applicable): not specified
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Composición Agua residual sintética (OCDE 209): Peptone 16 g; Meat extract 11 g; Urea 3 g; NaCl 0,7 g; CaCl2 2H2O 0,4 g; MgSO4 7H2O 0,2 g; K2HPO4 2,8 g diluted to 1000 mL with deionized water.
- Particulate matter: --
OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: not specified
- Details on termination of incubation: not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): respiration inhibition - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 338 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? yes, in the interval beteen 5mg/L and 30 mg/L, accepted range specified in the guideline OCDE 209
- Relevant effect levels: CE50 h = 14 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The result obtained in this study indicates that the test substance has a EC50(3h) of 338 mg/L.
- Executive summary:
The study of bacterial inhibition in activated sludge of the test substance was carried out, following the OECD Guideline 209 (1984) and Royal Decree 363/1995 (BOE No. 133 of June 1995).
This method is used to evaluate the effect of the test substance on microorganisms by measuring the respiration rate under certain conditions and in the presence of different concentrations of the substance
Activated sludge from an urban sewage treatment plant has been subjected to the dye at the test concentrations: 10, 32, 100, 320 and 1000 mg / L of active substance. The total duration of the test was 3 hours.
The result obtained in this study indicates that the test substance presents a value of EC50 (3h) equal to 338 mg / L of active material.
Reference
Table 1. pH values at the beginning of the study
concentration | control | 10 | 32 | 100 | 320 | 1000 | control |
pH | 7.36 | 7.36 | 7.36 | 7.35 | 7.29 | 7.17 | 7.36 |
Table 2. Oxygen measurements (mg/L) during the respiration inhibition test. Values were collected at 1 minute intervals
conc. mg/L | mg O2/L in the time interval (minutes) | ||||||||||
-- | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
control | 6.26 | 5.89 | 5.52 |
5.19 |
4.84 |
4.46 |
4.09 |
3.68 |
3.24 |
2.91 |
2.64 |
10 |
6.28 |
5.91 |
5.51 |
5.09 |
4.69 |
4.28 |
3.89 |
3.50 |
3.08 |
3.69 |
2.29 |
32 |
6.32 |
5.98 |
5.62 |
5.26 |
4.89 |
4.53 |
4.15 |
3.75 |
3.35 |
3.01 |
2.78 |
100 |
6.29 |
5.98 |
3.66 |
5.36 |
5.03 |
4.71 |
4.42 |
4.09 |
3.79 |
3.48 |
3.15 |
320 |
6.33 |
6.14 |
5.94 |
5.73 |
5.52 |
5.32 |
5.13 |
4.92 |
4.71 |
4.52 |
4.31 |
1000 |
6.28 |
6.22 |
6.15 |
6.08 |
5.99 |
5.93 |
5.85 |
5.78 |
5.70 |
5.64 |
5.56 |
control |
6.28 |
5.91 |
5.53 |
5.21 |
4.83 |
4.42 |
4.05 |
3.65 |
3.19 |
2.88 |
2.59 |
Table 3. Respiration rate (mg L-1 h-1) obtained at each of the concentrations and controls
control | 10 | 32 | 100 | 320 | 1000 | control | |
respiration rate mg/L.h | 21.72 | 23.94 | 21.24 | 18.84 | 12.12 | 4.32 | 22.14 |
Table 4. % respiration inhibition (3h)
Conc. (mg/L) |
% respiration inhibition (3h) |
control |
0 |
10 |
0 |
32 |
3 |
100 |
14 |
320 |
45 |
1000 |
80 |
Description of key information
The toxicity to aquatic microorganisms is determined by means of one test conducted on the substance Direct Red 254 TEA salt (CAS 64683-40 -5) according to OECD Guideline 209 that results on one EC50 338 mg/L value.
Based on the EC value obtained and considering the existing information, the substance is not harmful to microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 338 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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