2-aminotoluene-4-sulphonic acid

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from authorative database

Data source

Reference

Reference Type:

other: authorative database

Title:

Hazardous Substances Data Bank (HSDB) for 2-METHYL-1,4-BENZENEDIAMINE

Author:

U.S National Library of Medicine

Year:

2017

Bibliographic source:

Hazardous Substances Data Bank (HSDB) for 2-METHYL-1,4-BENZENEDIAMINE U.S National Library of Medicine 2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To evaluate eye irritation potential of 2,5-Di amino toluene on rabbit’s eye

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-methyl-p-phenylenediamine

EC Number:

202-442-1

EC Name:

2-methyl-p-phenylenediamine

Cas Number:

95-70-5

Molecular formula:

C7H10N2

IUPAC Name:

2-methylbenzene-1,4-diamine

Test material form:

solid

Details on test material:

-IUPAC name:2-methylbenzene-1,4-diamine
-Molecular formula :C7H10N2
-Molecular weight :122.17 g/mol
- Substance type:organic
- Physical state: solid
-InChI:1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
-Smiles:Cc1cc(ccc1N)N

Specific details on test material used for the study:

-IUPAC name:2-methylbenzene-1,4-diamine
-Molecular formula :C7H10N2
-Molecular weight :122.17 g/mol
- Substance type:organic
- Physical state: solid
-InChI:1S/C7H10N2/c1-5-4-6(8)2-3-7(5)9/h2-4H,8-9H2,1H3
-Smiles:Cc1cc(ccc1N)N

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

No data available

Test system

Vehicle:

other: aqueous sodium sulphite (pH 7.0)

Amount / concentration applied:

0.1 mL of a 2.5% w/v

Duration of treatment / exposure:

10 seconds

Observation period (in vivo):

1 hr , Days 1, 2, 3, 4, and 7.

Number of animals or in vitro replicates:

Total=3

Details on study design:

Washing (if done): eye was rinsed with 50 mL of lukewarm water
SCORING SYSTEM: Draize scoring system

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

7 d

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Mild conjunctival irritation was observed in 2 animals on days 1 and 3 respectively

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

2,5-Diaminotoluene was installed into the eye of three rabbits for in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. Mild conjunctival irritation was observed in 2 animals on days 1 and 3 respectively. Hence, 2,5-Diaminotoluene was considered to be slightly irritating to rabbit eye.

Executive summary:

2,5-Diaminotoluene was used as a test material to evaluate ocular irritation potential on three rabbits. The test material was installed onto the eyes of rabbits in the concentration 0.1 mL of a 2.5% w/v in aqueous sodium sulphite (pH 7.0) for 10 sec. After 10 seconds the eye was rinsed with 50 mL of lukewarm water.eyes were evaluated and scored by Draize scoring system at 1 hr and then at Days 1, 2, 3, 4, and 7.

Mild conjuctival irritation was observed in 2 animals on day 1 and 3 respectively . No data regarding reversibility of the irritation was stated. Under the conditions of this test, toluene-2,5-diamine solution caused slight irritation to rabbit eyes.