2-(diphenylphosphino)benzonitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Limit test.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 434 (Acute Dermal Toxicity - Fixed Dose Procedure)

Deviations:

yes

Remarks:

Method based on Noakes ansd Sanderson (1989)

Principles of method if other than guideline:

The acute (percutaneous) toxicity of the substance was determined using a method based on Noakes and Sanderson (1969)

2 male rats used (body weight 250-300g). A day prior to dosing, 60% of dorsal hair was shorn from each animal.

On day of dosing the skin are to be exposed was examined to rule out skin damage/micro abrasions.
The test material was applied to the skin usinga syringe and the area immediately occluded using plastic and waterproof adhesive tape.
A collar was placed on each animal to prevent interference with the pathed area.
Rats were individually caged for 7 hours and allowed ad libitum access to food and water.

After 7 hours, the collar and occusion were removed. The skin was washed with a warm solution of 0.5% TEGO, then warm water, then dried.
The rats were then rehoused in single-sex groups and allowed ad libitum food and water.

Animals were observed for 14 days after adminstration Body weights were measured on days 0, 7 and 14.
All surviving animals were destroyed on day 14.

GLP compliance:

not specified

Test type:

other: Noakes and Sanderson (1969)

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(diphenylphosphanyl)benzonitrile

Cas Number:

34825-99-5

Molecular formula:

C19H14NP

IUPAC Name:

2-(diphenylphosphanyl)benzonitrile

Test material form:

solid - liquid: suspension

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Bought from commercial supplier (Bantam & Kingman Ltd).
Body weight range 200-225 g.
On arrival, animals were housed in single sex groups of up to 5 per cage.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

corn oil

Details on dermal exposure:

On day of dosing the skin are to be exposed was examined to rule out skin damage/micro abrasions.
The test material was applied to the skin usinga syringe and the area immediately occluded using plastic and waterproof adhesive tape.
A collar was placed on each animal to prevent interference with the pathed area.
Rats were individually caged for 7 hours and allowed ad libitum access to food and water.

After 7 hours, the collar and occusion were removed. The skin was washed with a warm solution of 0.5% TEGO, then warm water, then dried.
The rats were then rehoused in single-sex groups and allowed ad libitum food and water.

Duration of exposure:

7 hours followed by 14 day observation period.

Doses:

200 mg/kg/body weight

No. of animals per sex per dose:

2 males

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The dermal LD50 of the tested substance is >2000 mg/kg/body weight. No overt signs of toxicity observed.