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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
Remarks:
The study was performed in accordance with the study plan and guideline (OECD TG 201), and no deviation took place during the test period.
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Test substance : Ethoxylated-o-phenylphenol acrylate
Lot No : 71016M95
Receipt date : 2007-11-12
Receipt quantity : 200 mL
Appearance : Clear liquid
Purity : 97.4 %
Solubility : Water – insoluble,
DMSO, Acetone, Ethanol - soluble
pH : -
Storage condition : room temperature
Supplier : Miwon Commercial Co., Ltd
Vehicle : Tween(R) 80(Junsei, Lot No. 6A4007)
Analytical monitoring:
yes
Details on test solutions:
A stock solution was prepared by weighing out 0.5133 g test substance, then made it up to 100 mL with TweenⓇ 80 (0.5 g) to give 5000 mg/L.
0.100, 0.250, 0.626, 1.562, 3.906 and 9.766 mL of stock solution were transferred into 10 mL of volumetric flasks and filled up to the 10 mL marker with TweenⓇ 80 : ground water (0.5:99.5, w/v) in test water to prepare working solutions.
5 mL of each working solution was added test water and filled up to the 5 L marker to prepare test solutions.
Solvent concentration (Tween(R) 80) of each test solution was 50 mg/L.
The range-finding test was performed in 0.1, 1.0, 10.0 and 100.0 mg/L. The inhibition rate for the average specific growth rate was –2.1, -8.8, 64.2 and 97.0 %, respectively.
The inhibition rate for the yield was 13.4, 18.0, 90.8 and 99.6 %, respectively.
The definitive test was performed in 0.50, 1.25, 3.13, 7.81, 19.53 and 48.83 mg/L (nominal concentration, geometric series 2.5).
As analysis result of test substance in test solution, the measured mean concentrations were 0.42, 1.02, 2.38, 6.09, 14.48 and 41.00 mg/L, respectively.

Negative control : OECD medium was used for negative control.
Solvent control : 50 mg/L of TweenⓇ80 (in OCED medium) was used for solvent control.
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
Freshwater alga (Pseudokirchneriella subcapitata , ATCC22662).
The culturing vessels were shaken and placed in the culturing apparatus (J-SCI, Jisico Co., Korea). The cultures were maintained at a temperature in the range of 21oC - 24oC. The surface of cultures were received continuous, uniform fluorescent illumination in the range of 4440 Lux - 8880 Lux. The culturing vessels were 250 mL of glass flasks.
The temperature in the incubator was recorded automatically every 1 h using TR-71U thermo-recorder (TND Co., Japan). The light intensities were measured in the incubator using ANA-11 digital lux meter (Tokyo Koden Co., Japan) twice a week. As the results of the monitoring, there was not observed an alteration effecting the test.
The growth medium was prepared by OECD medium (OECD TG 201, Annex 3 Growth media, 2006-03-23). The pH values of OECD medium was measured using PP-15 pH meter (Sartorius AG, Germany).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
The mean temperature in the incubator was 22.7oC (min. 22.1oC - max. 23.6oC)
pH:
mean pH was 8.22 ± 0.30 (min. 7.84 - max. 8.60)
Nominal and measured concentrations:
The definitive test was performed with 0.50, 1.25, 3.13, 7.81, 19.53 and 48.83 mg/L (nominal concentration, geometric series 2.5). The analysis of test concentrations were performed at the start of the test and the end of the test.
The analysis results of test substance in the test solution was 0.42, 1.02, 2.38, 6.09, 14.48 and 41.00 mg/L.
Details on test conditions:
The pre-culture was incubated under the test condition and used when exponentially growing, after incubation period of 4 days. The pre-cultured alga was inoculated (1 ×
10^4) cells/mL in each vessel. And each test concentration has 3 replicates. The test vessels were placed in the shaking incubator (J-SCI, Jisico Co., Korea) maintained at the temperature of 21oC - 24oC, the light intensity of 4440 Lux - 8880 Lux, always light and 100 rpm. The test vessels were 250 mL of glass flasks.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
2.38 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
9.791 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
4.097 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
1.02 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
act. ingr.
Basis for effect:
other: Yield
Results with reference substance (positive control):
Following the test results of Potassium dichromate (K2Cr2O7, Sigma-Aldrich Inc., Lot No. 09514PA) to freshwater alga, the 72 h-ErC50 was determined as 0.275 mg/L and 95% confidence limits was determined as 0.253 mg/L - 0.299 mg/L. The 72 h-EyC50 value was determined as 0.232 mg/L, and 95 % confidence limits was determined as 0.217 mg/L ~ 0.249 mg/L.
Reported statistics and error estimates:
72 h-ErC10 was 2.237 mg/L (95 % confidence interval : 1.716 mg/L – 2.772 mg/L)
72 h-ErC50 was 9.791 mg/L (95% confidence interval : 8.375 mg/L – 11.548 mg/L).
The NOEC was 2.38 mg/L.
72 h-EyC10 was 1.087 mg/L (95% confidence interval :0.850 mg/L – 1.330 mg/L)
72 h-EyC50 was 4.097 mg/L (95% confidence interval : 3.552 mg/L – 4.730 mg/L).
The NOEC was 1.02 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
NOEC and EC50 values for the toxicity of to the freshwater green algae Pseudokircheneriella subcapitata was established at 72 hours, both in terms of yield and growth rate according to OECD 201. NOEC (72h) were 2.38 and 1.02 mg/l, and EC50 (72h) were 9.79 and 4.10 mg/L, when based on growth rate and yield, respectively
Executive summary:

NOEC and EC50 values for the toxicity of to the freshwater green algae Pseudokircheneriella subcapitata was established at 72 hours, both in terms of yield and growth rate according to OECD 201. NOEC (72h) were 2.38 and 1.02 mg/l, and EC50 (72h) were 9.79 and 4.10 mg/L, when based on growth rate and yield, respectively.

Description of key information

NOEC and EC50 values for the toxicity of  Biphenyl-2 -ol, ethoxylated, esters with acrylic acid to the freshwater green algae Pseudokircheneriella subcapitata was established at 72 and 96h hours, both in terms of biomass and growth rate.

Key value for chemical safety assessment

EC50 for freshwater algae:
9.79 mg/L
EC10 or NOEC for freshwater algae:
2.62 mg/L

Additional information

NOEC and EC50 values for the toxicity of to the freshwater green algae Pseudokircheneriella subcapitata was established at 72 hours, both in terms of yield and growth rate according to OECD 201. NOEC (72h) were 2.38 and 1.02 mg/l, and EC50 (72h) were 9.79 and 4.10, when based on growth rate and yield, respectively.

No data on marine algae have been identified.