Di-tert-butyl 1,1,4,4-tetramethylbut-2-yn-1,4-ylene diperoxide

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1964

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1964

Report date:

1964

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Di-tert-butyl 1,1,4,4-tetramethylbut-2-yn-1,4-ylene diperoxide

EC Number:

213-944-5

EC Name:

Di-tert-butyl 1,1,4,4-tetramethylbut-2-yn-1,4-ylene diperoxide

Cas Number:

1068-27-5

Molecular formula:

C16H30O4

IUPAC Name:

2,5-bis(tert-butylperoxy)-2,5-dimethylhex-3-yne

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: the test compound was received from Lucidol Division, Wallace and Tiernan, Inc.
- Weight at study initiation: 1729 to 2504 g
- Housing: All animals were placed in holders designed to immobilize the animal

Test system

Type of coverage:

occlusive

Preparation of test site:

other: shaved and abraded

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied:0.5 mL/rabbit

Duration of treatment / exposure:

24 hours

Observation period:

72 hours

Number of animals:

twelve albino rabbits (two groups of 6 animals)

Details on study design:

TEST SITE
- Area of exposure: Approximately one inch square on each site of the back of the animals.
- Type of wrap if used: The rabbits were further prepared following application by placing a piece of rubber dam 2x2 inches over each area of application. The damming was held in place with adhesive tape and the entire trunk of each animal wrapped with rubberized cloth bandage to hold the patches in place and retard the evaporation of volatile substance.

REMOVAL OF TEST SUBSTANCE
- Washing: The back of each rabbit was washed with lukewarm water
- Time after start of exposure: 24 hours

READINGS: The areas of compound application were exermined after 24 hours and 72 hours

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

On the basis of the results obtained in this study, the test substance does not possess any primary irritating properties to the skin of albino rabbits under the test conditions chosen.

Executive summary:

The test substance was applied once only at a total volume of 0.5 mL/rabbit to the prepared dermal surface of the back of albino rabbits. On the basis of the results obtained in this study, the test substance dies not possess any primary irritating properties to the skin of this species under the test conditions chosen (mean erythema score and mean edema score = 0).