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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Range-Finding Toxicity Data: List VIII.
Author:
Carpenter CP et al.
Year:
1974
Bibliographic source:
Toxicol. Appl. Pharmacol. 28: 313-319

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Range-finding toxicity test according to the method described by Smyth HF Jr. et al. (1962).
Smyth HF Jr. et al. (1962). Range-finding toxicity data: List VI. Amer. Ind. Hyg. Ass. J. 23: 95-107
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Butane-1,3-diyl diacrylate
EC Number:
243-105-9
EC Name:
Butane-1,3-diyl diacrylate
Cas Number:
19485-03-1
Molecular formula:
C10H14O4
IUPAC Name:
butane-1,3-diyl bisacrylate
Details on test material:
no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: 4-5 weeks
- Weight at study initiation: 90-120 g
- Fasting period before study: no


Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Doses:
The dosages were arranged in a logarithmic series differing by a factor of two.
No. of animals per sex per dose:
6 males
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Test conditions and method according to Smyth et al. (1962):
Single oral dose toxicity was estimated by the gastric intubation of groups of five non-fasted, Carworth-Wistar male rats, or in rare instances of female rats, four to five weeks of age and 90 to 120 grams. The dosages were arranged in a logarithmic series differing by a factor of two. Whenever possible, the chemical was administered undiluted. When a lesser concentration was necessary, solution in water or corn oil or suspension in semi-solid agar were the preferred expedients. Occasionally, a 1 % solution of Tergitol penetrant 7 (essentially an aqueous solution of 25 % sodium 3,9-diethyl-6-tridecanol sulfate) has been used as a dispersing agent. Based upon mortalities during a 14-day observation period, the most probable LD50 value and its fiducial range were estimated by the method of Thompson (1947).

Statistics:
The most probable LD50 value and its fiducial range were estimated by the method of Thompson (1947).

Thompson WR (1947). Use of Moving Averages and Interpolation to Estimate  Median Effective Dose. Bacteriol. Rev. 11: 115

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
3.45 mL/kg bw
Based on:
test mat.
95% CL:
> 2.57 - < 4.87
Remarks on result:
other: with a density of 1.037, the LD50 is 3577 mg/kg bw
Mortality:
yes, no details
Clinical signs:
no data
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
Based on the results, the oral LD50 of 1,3-butylene glycol diacrylate is 3577 mg/kg (corresponding to 3.45 ml/kg bw) in rats.