Sodium 4-chloro-3-nitrobenzenesulphonate

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on daphnia magna was estimated for test chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate (CASno. 17691-19-9). The EC 50 value of test chemical  sodium 4-chloro-3-nitrobenzene-1-sulfonate was estimated to be 150.137 mg/L when it was exposed to Daphnia magna for 48 hrs. On the basis of EC50 value it is concluded that this test chemical is considered as not classified according to CLP criteria.

Toxicity to aquatic algae and cyanobacteria :

Toxicity of test chemical Sodium 4-chloro-3-nitrobenzenesulphonate (CAS no. 17691-19-9) was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the five closest read across substances. When test chemical Sodium 4-chloro-3-nitrobenzenesulphonate was exposed for 72 hrs on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) alga, EC50 value was estimated to be 161.105 mg/L. Since EC50 value is greater than 100 mg/L it is concluded that this test chemical can be considered to be not classified according to CLP classification criteria.

Additional information

Short term toxicity to aquatic invertebrates:

To determine short term toxicity to aquatic invertebrates of test chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate (CAS no. 17691 -19 -9) various studies have been carried out in these studies one study is predicted data study for target chemical and three studies are experimental studies of target chemical’s structurally similar read across chemicals to corroborate findings of target chemicals and results of all the studies are summarized below.

The first weight of evidence study was predicted data study for target chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate in this study the prediction was done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on daphnia magna was estimated for test chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate. The EC 50 value of test chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate was estimated to be 150.137 mg/L when it was exposed to Daphnia magna for 48 hrs. On the basis of EC50 value it is concluded that this test chemical is considered as not classified according to CLP criteria.

Next study was experimental study done from ABITEC laboratory 2016, for structurally similar read across chemical 3 -Nitrobenzene sulfonic acid in this study the determination of the inhibition of the mobility of daphnids was carried out with the read across substance 3 -Nitrobenzene sulfonic acid according to OECD Guideline 202. The read across substance was tested at the limit concentration 100 mg/l. Effects on immobilization were observed for 48 hours. Based on the % inhibition of daphnia magna, due to the exposure of chemical 3 -Nitrobenzene sulfonic acid, the IC8 was 100 mg/l. This value indicates that the read across substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as toxic as per the CLP criteria.

Further, another experimental study have been carried out to determine Short term toxicity of structurally similar read across chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate in Daphnia magna which were cultured in laboratory from starting culture originally obtained from Put in Bay, Ohio. The experiment was static test and medium used was glasswool filtered university lake water. Median tolerance limit (TLm) of read across chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate was observed in daphnia magna at different time intervals. At 24 hrs and 48 hrs it was 8665 mg/L and at 72 hrs TLm was 6017 mg/L on the basis of immobilization. On the basis of TLm values at different time interval it is concluded that this read across chemical is considered as not classified according to CLP criteria.

 

Last experimental study was done from ECOTOX knowledgebase, database (2017) for target chemical’s closest read across chemical 2,4,6-trinitrophenol with log Kow as primary descriptor. In this study the short term toxicity of 2,4,6-trinitrophenol on the growth and mobility of aquatic invertebrate daphnia magna was determined by using 6-24 hrs newborn Daphnia . Static method was performed in which daphnias were exposed for 24hrs with the read across chemical 2,4,6-trinitrophenol. After the exposure of chemical for 24hrs, immobilization was observed at the concentration at which 50% daphnia killed. Based on the immobilization of daphnia magna due to the exposure of chemical for 24hrs, the EC50 was 104 mg/l. Based on the EC50 value, it can be concluded that the read across chemical was nontoxic and can be consider to be not classified as toxic as per the CLP classification criteria.

By compiling all above mentioned studies from different sources of target chemical sodium 4-chloro-3-nitrobenzene-1-sulfonate (CAS no. 17691 -19 -9) and its read across chemicals it is concluded that target chemical can be considered as non toxic and not classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria :

Predicted data for target chemical 4-chloro-3-nitrobenzenesulphonate (CAS no. 17691-19-9) and experimental studies for its structurally similar read across chemical are summarized below for toxicity to aquatic algae endpoint.

In first weight of evidence study the toxicity of test chemical Sodium 4-chloro-3-nitrobenzenesulphonate (CAS no. 17691-19-9) was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the five closest read across substances. When test chemical Sodium 4-chloro-3-nitrobenzenesulphonate was exposed for 72 hrs on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) alga, EC50 value was estimated to be 161.105 mg/L.

Next study was experimental study done from ABITEC laboratory 2016  for structurally similar read across chemical in this study freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the read across substance 3-Nitrobenzene sulfonic acid according to OECD Guideline 201. The read across substance was dissolved in OECD growth medium and tested at the limit concentration 100 mg/L and Effects on the growth rate of the organism were studied. 

The NOEC for the read across substance, 3-Nitrobenzene sulfonic acid, sodium salt, in Desmodesmus subspicatus was determined to be 100 mg/L on the basis of effects on growth rate in a 72 hour study. This value indicates that the read across substance is likely to be non-hazardous to aquatic algae and cannot be classified as toxic as per the CLP criteria.

Another experimental study for Short term toxicity to aquatic algae endpoint was done from Ecotox knowledgebase, database 2017. Toxicity to aquatic algae i.e., Pseudokirchneriella subcapitata (Green Algae) was determined by exposing it to test chemical Mono Nitrobenzene (CAS no. 98-95-3) for 24 hrs. On the basis of biochemistry of Pseudokirchneriella subcapitata (Chlorophyll A concentration) EC 50 value was determined to be 100 mg/L. By considering EC 50 value of read across chemical Mono Nitrobenzene it is concluded that this read across chemical is considered as not classified according to CLP classification criteria.

Based on the results of all above studies for test chemical Sodium 4-chloro-3-nitrobenzenesulphonate (CAS no. 17691-19-9) it is concluded that target chemical can be considered as toxic to green algae at environmentally relevant concentrations and can be considered as not classified per the CLP classification criteria.