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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
data is from experimental reports

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Acute Oral toxicity test was carried out to study the effects of Sodium 4-chloro-3-nitrobenzenesulphonate (17691-19-9) on rats.
GLP compliance:
not specified
Test type:
other: no data available
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium 4-chloro-3-nitrobenzenesulphonate
EC Number:
241-680-0
EC Name:
Sodium 4-chloro-3-nitrobenzenesulphonate
Cas Number:
17691-19-9
Molecular formula:
C6H3ClNNaO5S
IUPAC Name:
sodium 4-chloro-3-nitrobenzene-1-sulfonate
Details on test material:
- Name of test material (IUPAC name): Sodium 4-chloro-3-nitrobenzenesulphonate
- Common name: 4-Chloro-3-nitrobenzenesulfonic acid, sodium salt
- Molecular formula: C6H3ClNNaO5S
- Molecular weight: 259.601 g/mol
- Smiles notation: S(=O)(=O)([O-])c1cc([N+]([O-])=O)c(Cl)cc1.[Na+]
- InChl: 1S/C6H4ClNO5S.Na/c7-5-2-1-4(14(11,12)13)3-6(5)8(9)10;/h1-3H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
Specific details on test material used for the study:
- Name of test material (as cited in study report):sodium 4–chloro–3–nitrobenzenesulphonate
- Molecular formula :C6H4NNaO5S
- Molecular weight :225.1556 g/mol
- Substance type:organic

Test animals

Species:
rat
Strain:
other: Wistar (SPF cpB)
Sex:
male/female
Details on test animals or test system and environmental conditions:
Details on test animal:
TEST ANIMALS:
- Source: winkelmann, Borchen
- Age at study initiation: 14 weeks old avg.
- Weight at study initiation: Initial weight of male animals was 161 g and the female rats had an average initial weight of 160 g.
- Fasting period before study: No data available
- Housing: The rats were kept conventionally in Makrolonkaefigen type III on dust-free wood granules in groups of 5 each animals.
- Diet (e.g. ad libitum): the feed : Altromin R 1324 was given ad libitum
- Water (e.g. ad libitum): water was provided ad libitum for approximately 16 hours and before until about 4 hours after application.
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1.5 degrees Celsius
- Humidity (%):approx. 60 +/- 5%
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): 2 hours of light / dark rhythm (artificial Lighting from 7 am to 7 pm CET)

IN-LIFE DATES: From: To: 25.8.1980 to 8.9.1980

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
water
Details on oral exposure:
Details on exposure:
VEHICLE
- Concentration in vehicle:5000mg/kg bw
- Amount of vehicle (if gavage): 20 ml/kg bw

- Justification for choice of vehicle: test substance was soluble in water

DOSAGE PREPARATION (if unusual): The substance was formulated in water using a rigid metal stomach probe in koaslantem
Doses:
5000 mg/kg bw
No. of animals per sex per dose:
Total : 10 animals
5000 mg/kg bw :5 males and 5 females
Control animals:
not specified
Details on study design:
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of weighing: after 1 week and at the end of the
14 days animals were weighed individually.
- Necropsy of survivors performed: yes
- Other examinations performed: Clinical signs
Statistics:
No data available

Results and discussion

Preliminary study:
No data available
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No mortality was observed in treated rats at 5000 mg/kg bw
Mortality:
No mortality was observed in treated rats at 5000 mg/kg bw
Clinical signs:
No poisoning symptoms were observed
Body weight:
No data available
Gross pathology:
The section of some animals killed at the end of the experiment showed no macroscopic findings.
Other findings:
No data available

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
The lethal concentration (LD50) value for acute oral toxicity test was considered to be >5000 mg/kg bw ,when 5 male and 5 female Wistar (SPF cpB) rats were treated with Sodium 4-chloro-3-nitrobenzenesulphonate (17691-19-9) orally.
Executive summary:

Acute oral toxicity study was done in 5 male and 5 female Wistar (SPF cpB)rats using test material Sodium 4-chloro-3-nitrobenzenesulphonate(17691-19-9) following 14 days of observation period.Rats were 14 weeks old.Initial weight of male animals was 161 g and the female rats had an average initial weight of 160 g. The rats were conventionally kept in Makrolonkaefigen type III on dust-free wood granules at a room temperature of 22 +/- 1.5 degrees Celsius and 2 hours of light / dark rhythm (artificial lighting from 7 am to 7 pm CET) and a relative humidity of approx. 60 +/- 5% in groups of 5 each animals.The feed,Altromin R 1324 was given ad libitum. Water was provided ad libitum for approximately 16 hours and before until about 4 hours after application.Water was used as vehicle.The substance was formulated in water using a rigid metal stomach probe in koaslantem. Application volume (20 ml / kg body weight) to each 5

males and 5 females were given once. After 1 week and at the end of the 14 days animals were weighed individually.No mortality was observed at dose 5000 mg/kg bw.No poisoning symptoms were observed in animals.The section of some animals killed at the end of the experiment showed no macroscopic findings.Hence,LD50 value was considered to be >5000 mg/kg bw,when rats were treated withSodium 4-chloro-3-nitrobenzenesulphonate(17691-19-9) orally.