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EC number: 241-680-0 | CAS number: 17691-19-9
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- data is from experimental reports
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- The objective of the study was to determine the ocular toxicity of Sodium 4-chloro-3-nitrobenzenesulphonate
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Sodium 4-chloro-3-nitrobenzenesulphonate
- EC Number:
- 241-680-0
- EC Name:
- Sodium 4-chloro-3-nitrobenzenesulphonate
- Cas Number:
- 17691-19-9
- Molecular formula:
- C6H3ClNNaO5S
- IUPAC Name:
- sodium 4-chloro-3-nitrobenzene-1-sulfonate
- Test material form:
- solid
- Details on test material:
- - Name of the test material: Sodium 4-chloro-3-nitrobenzenesulphonate
- IUPAC Name : sodium 4-chloro-3-nitrobenzene-1-sulfonate
- Molecular formula: C6H4ClNO5SNa
- Molecular weight: 259.601 g/mol
- Smiles: S(=O)(=O)([O-])c1cc([N+]([O-])=O)c(Cl)cc1.[Na+]
- InChI: 1S/C6H4ClNO5S.Na/c7-5-2-1-4(14(11,12)13)3-6(5)8(9)10;/h1-3H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid powder (Off white to pale yellow)
Constituent 1
- Specific details on test material used for the study:
- - Name of the test material: Sodium 4-chloro-3-nitrobenzenesulphonate
- IUPAC Name : sodium 4-chloro-3-nitrobenzene-1-sulfonate
- Molecular formula: C6H4ClNO5SNa
- Molecular weight: 259.601 g/mol
- Smiles: S(=O)(=O)([O-])c1cc([N+]([O-])=O)c(Cl)cc1.[Na+]
- InChI: 1S/C6H4ClNO5S.Na/c7-5-2-1-4(14(11,12)13)3-6(5)8(9)10;/h1-3H,(H,11,12,13);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid powder (Off white to pale yellow)
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Sex: Male and female
- Source: Hacking and Churchill Limited, Huntingdon, England
- Age at study initiation: no data available
- Weight at study initiation:
- Housing:individually in stainless steel cages
- Diet (e.g. ad libitum): Ssniff K Pallets, ad libitum (Ssniff Experimental animal diet GmbH, 4770 Soest)
- Water (e.g. ad libitum): Drinking water ad libitum from plastic bottles with steel nipples
- Acclimation period: no data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 deg C
- Humidity (%): 60%
- Air changes (per hr): no data available
- Photoperiod (hrs dark / hrs light):12-hour light-dark rhythm
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 100mg/rabbit
- Duration of treatment / exposure:
- single exposure
- Observation period (in vivo):
- The observation period lasted 72 hours or 7 days. Each animal was observed at 1h, 24, 48, 72 hours and, if necessary, 7 days after treatment fro ocualr lesions
- Duration of post- treatment incubation (in vitro):
- no data available
- Number of animals or in vitro replicates:
- 3 (1 male, 2 female)
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data available
- Time after start of exposure: no data available
SCORING SYSTEM: The ocular lesions were scored according to the following OECD Guidelines where appropriate responses were recorded
TOOL USED TO ASSESS SCORE: hand-slit lamp / biomicroscope / fluorescein: For the eye examination a hand slit lamp was used.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness and swelling
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- No special observations were done.
Any other information on results incl. tables
Table: Acute Ocular Irritation in Rabbits
(According to OECD 405 Guidelines)
Rabbit number |
Sex |
Cornea |
Iris |
Conjunctiva (R/S)* |
Total |
||||||||||||||||
1h |
24h |
48h |
72h |
7 days |
1h |
24h |
48h |
72h |
7 days |
1h |
24h |
48h |
72h |
7 days |
1h |
24h |
48h |
72h |
7 days |
||
135 |
M |
|
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
|
0/0 |
1/0 |
0/0 |
0/0 |
|
0 |
1 |
0 |
0 |
166 |
F |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/0 |
1/0 |
1/0 |
1/0 |
0/0 |
0 |
1 |
1 |
1 |
0 |
176 |
F |
|
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
|
0/0 |
1/0 |
1/0 |
0/0 |
|
0 |
0 |
0 |
0 |
Mean |
|
0 |
0 |
0 |
0 |
0 |
|
0 |
0 |
0 |
0 |
|
0/0 |
1/0 |
1/0 |
0/0 |
|
0 |
1 |
1 |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating
- Conclusions:
- No special observations were observed in rabbits after 7 days. The mean irritation score for cornea, conjunctivae (redness and swelling) and iris after 7 days were 0,0,0 respectively.
Based on the scores and observations, Sodium 4-chloro-3-nitrobenzenesulphonate can be considered to be not irritating to rabbit eyes. - Executive summary:
The objective of the study was to determine the ocular toxicity of Sodium 4-chloro-3-nitrobenzenesulphonate. The study was performed as per OECD 405 Guidelines. 3 New Zealand White rabbits(2 female and 1 male) were used for the study. 100 mg of the test material / rabbit was placed in the eyelid pocket of one eye created by peeling off the lower eyelid. The second eye remained untreated and served as a control. For the eye examination a hand slit lamp was used.The observation period lasted 72 hours or 7 days. The observation period lasted 72 hours or 7 days. Each animal was observed at 1h, 24, 48, 72 hours and, if necessary, 7 days after treatment for ocular lesions.
The ocular lesions were scored according to the following OECD Guidelines where appropriate responses were recorded.
No special observations were observed in rabbits after 7 days. The mean irritation score for cornea, conjunctivae (redness and swelling) and iris after 7 days were 0,0,0 respectively.
Based on the scores and observations, Sodium 4-chloro-3-nitrobenzenesulphonate can be considered to be not irritating to rabbit eyes.
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