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EC number: 278-387-2 | CAS number: 76186-07-7
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Long-term toxicity to aquatic invertebrates
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from j -check
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- In vitro genetic toxicity study for 4-tert-octylphenol
- Author:
- National Institute of Technology and Evaluation.
- Year:
- 2 017
- Bibliographic source:
- Bibliographic source: Japan chemicals collaborative knowledge database (J-check), 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of 4-Nitro-m-cresol in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and E. coli WP2 uvrA by reverse mutation assay.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-(1,1,3,3-tetramethylbutyl)phenol
- EC Number:
- 205-426-2
- EC Name:
- 4-(1,1,3,3-tetramethylbutyl)phenol
- Cas Number:
- 140-66-9
- Molecular formula:
- C14H22O
- IUPAC Name:
- 4-(1,1,3,3-tetramethylbutyl)phenol
Constituent 1
- Specific details on test material used for the study:
- Details on test material
- Name of test material (as cited in study report): 4-tert-octylphenol
- Molecular formula: C14H22O
- Molecular weight: 206.32g/mol
- Substance type: Organic
-Purity: > 97%
Method
- Target gene:
- Histidine for Salmonella typhimurium and tryptophan Escherichia coli
Species / strain
- Species / strain / cell type:
- bacteria, other: Salmonella typhimurium TA100, TA1535, TA98, TA1537 and E. coli WP2 uvrA
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- S. typhimurium: 0,1.56, 3.13, 6.25, 12.5, 25, 50, 100, 200 µg/plate
E. coli: 0, 125, 250, 500, 1000, 2000 µg/plate - Vehicle / solvent:
- Vehicle
- Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: -S9; AF-2 (TA100, WP2, TA98), sodium azide (TA1535) and 9- aminoacridine (TA1537) +S9;2-aminoanthracene (all strains)
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: Plate method
NUMBER OF REPLICATIONS: Duplicate
OTHER EXAMINATIONS: 3 plates per test were observed. - Rationale for test conditions:
- Not specified.
- Evaluation criteria:
- Evaluation was done considering a dose dependent increase in the number of revertants/plate.
- Statistics:
- Not specified.
Results and discussion
Test results
- Species / strain:
- bacteria, other: Salmonella typhimurium TA100, TA1535, TA98, TA1537 and E. coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Additional information on results
Minimum concentration of test substance at which toxicity to bacteria was observed:
with metabolic activation: 200 µg/plate
without metabolic activation: 25 µg/plate - Remarks on result:
- other: No mutagenic effect were observed.
Applicant's summary and conclusion
- Conclusions:
- 4-tert-octylphenol (140-66-9) was evaluated for its mutagenic potential in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and E. coli WP2 uvrA by AMES test. The test result was considered to be negative in all strain in the presence and absence of metabolic activation S9.
- Executive summary:
Genetic toxicity in vitro study was assessed for 4-tert-octylphenol. For this purpose bacterial reverse mutation assay was performed according to Guidelines for Screening Mutagenicity Testing of Chemicals(Japan).The test material was exposed to Salmonella typhimurium TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA in the presence and absence of metabolic activation S9. The concentration of test material used in the presence and absence of metabolic activation were S. typhimurium: 0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 200 µg/plate, E. coli: 0, 125, 250, 500, 1000, 2000 µg/plate. No mutagenic effects were observed in all strains, in the presence and absence of metabolic activation. Therefore 4-tert-octylphenol was considered to be non mutagenic in Salmonella typhimurium TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA by AMES test. Hence the substance cannot be classified as gene mutant in vitro.
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