Essential Oil of Piper nigrum L. (Family: Piperaceae). Obtained by the steam distillation of dried unripe berries.

Endpoint summary

Description of key information

Skin irritation :

The dermal irritation potential of Black pepper oil was estimated using OECD QSAR toolbox v3.3 with logPow as the primary descriptor.

Black pepper oil was estimated to be not irritating to the skin of New Zealand White rabbits.

Based on the estimated results, Black pepper can be considered to be not irritating to skin and can be classified under the category “Not Classified” as per CLP regulation.

Eye irritation:

The ocular irritation potential of Black pepper oil was estimated using OECD QSAR toolbox v3.4 with logPow as the primary descriptor.

Black pepper oil was estimated to be irritating to the eye of New Zealand White rabbits.

Based on the estimated results, Black pepper can be considered to be irritating to eye and can be classified under the category “ Category 2 irritating to eye” as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is predicted using OECD QSAR toolbox version 3.3 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: estimated data

Principles of method if other than guideline:

Prediction was done by using OECD QSAR toolbox v3.3

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC name: Pepper, black, oil
- Molecular formula: Unspecified
- Molecular weight: Unspecified
- Smiles : Unspecified
- Inchl: Unspecified
- Substance type: Organic
- Physical state: Liquid (colorless to slightly greenish)

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

not data available

Type of coverage:

not specified

Preparation of test site:

not specified

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

not data available

Duration of treatment / exposure:

not data available

Observation period:

not data available

Number of animals:

not data available

Details on study design:

not data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: not specified

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No indication of skin irritation estimated

Estimation method: Takes mode value from the 6 nearest neighbours
Domain  logical expression:Result: In Domain

((((((((("a" or "b" or "c" )  and "d" )  and ("e" and ( not "f") )  )  and "g" )  and "h" )  and "i" )  and "j" )  and "k" )  and ("l" and "m" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Alkene OR Allyl OR Cycloalkene by Organic Functional groups ONLY

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Allyl OR Cycloalkene OR Overlapping groups by Organic Functional groups (nested) ONLY

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as Aliphatic Carbon [CH] OR Aliphatic Carbon [-CH2-] OR Aliphatic Carbon [-CH3] OR Olefinic carbon [=CH- or =C<] OR Olefinic carbon [=CH2] OR Tertiary Carbon by Organic functional groups (US EPA) ONLY

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as No alert found by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Non binder, MW>500 OR Non binder, non cyclic structure by Estrogen Receptor Binding

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Class 1 (narcosis or baseline toxicity) by Acute aquatic toxicity classification by Verhaar (Modified) ONLY

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Low (Class I) by Toxic hazard classification by Cramer (with extensions) ONLY

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Bioavailable by Lipinski Rule Oasis ONLY

Domain logical expression index: "j"

Similarity boundary:Target: CC(=C)C1CCC(C)=CC1
Threshold=10%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "k"

Similarity boundary:Target: CC(=C)C1CCC(C)=CC1
Threshold=30%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "l"

Parametric boundary:The target chemical should have a value of log Kow which is >= 3.59

Domain logical expression index: "m"

Parametric boundary:The target chemical should have a value of log Kow which is <= 4.92

Interpretation of results:

other: Not irritating

Conclusions:

Black pepper oil was considered not irritating to New Zealand Rabbit's skin

Executive summary:

The dermal irritation potential of Black pepper oil was estimated using OECD QSAR toolbox v3.3 with logPow as the primary descriptor.

Black pepper oil was estimated to be not irritating to the skin of New Zealand White rabbits.

Based on the estimated results, Black pepper can be considered to be not irritating to skin and can be classified under the category “Not Classified” as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is predicted using OECD QSAR toolbox version 3.4 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: estimated data

Principles of method if other than guideline:

Prediction was done by using OECD QSAR toolbox v3.4

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Black Pepper Oil
- IUPAC name: Pepper, black, oil (Piper nigrum L.)
- Molecular formula: Unspecified
- Molecular weight: Unspecified
- Smiles : Unspecified
- Inchl: Unspecified
- Substance type: Organic
- Physical state: Liquid (colorless to slightly greenish)

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

No data available

Vehicle:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

No data available

Observation period (in vivo):

48 hrs

Number of animals or in vitro replicates:

No data available

Details on study design:

No data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

48 h

Reversibility:

not reversible

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

positive indication of irritation estimated

Estimation method: Takes mode value from the 7 nearest neighbours
Domain  logical expression:Result: In Domain

((((((((((((((((((((((((((((((((((("a" or "b" or "c" )  and ("d" and ( not "e") )  )  and ("f" and ( not "g") )  )  and ("h" and ( not "i") )  )  and ("j" and ( not "k") )  )  and ("l" and ( not "m") )  )  and ("n" and ( not "o") )  )  and ("p" and ( not "q") )  )  and ("r" and ( not "s") )  )  and ("t" and ( not "u") )  )  and ("v" and ( not "w") )  )  and "x" )  and "y" )  and "z" )  and "aa" )  and "ab" )  and "ac" )  and ("ad" and ( not "ae") )  )  and ("af" and ( not "ag") )  )  and ("ah" and ( not "ai") )  )  and ("aj" and ( not "ak") )  )  and "al" )  and ("am" and ( not "an") )  )  and ("ao" and ( not "ap") )  )  and ("aq" and ( not "ar") )  )  and ("as" and ( not "at") )  )  and ("au" and ( not "av") )  )  and ("aw" and ( not "ax") )  )  and ("ay" and ( not "az") )  )  and ("ba" and ( not "bb") )  )  and ("bc" and ( not "bd") )  )  and "be" )  and "bf" )  and ("bg" and ( not "bh") )  )  and ("bi" and "bj" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Alkene OR Allyl OR Cycloalkene OR Terpenes by Organic Functional groups ONLY

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as Aliphatic Carbon [CH] OR Aliphatic Carbon [-CH2-] OR Aliphatic Carbon [-CH3] OR Olefinic carbon [=CH- or =C<] OR Olefinic carbon [=CH2] OR Tertiary Carbon by Organic functional groups (US EPA) ONLY

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Moderate binder, NH2 group OR Non binder, impaired OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Non binder, MW>500 by Estrogen Receptor Binding

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Strong binder, NH2 group OR Strong binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as (!Undefined)Group CN Lipid Solubility < 0.4 g/kg OR (!Undefined)Group CNHal Lipid Solubility < 400 g/kg OR (N/A) by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "m"

Referential boundary: The target chemical should be classified as Group All Aqueous Solubility < 0.000005 g/L by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "o"

Referential boundary: The target chemical should be classified as Group All Aqueous Solubility < 0.00002 g/L OR Group All log Kow < -3.1 by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "p"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "q"

Referential boundary: The target chemical should be classified as Group CHal log Kow > 4.5 OR Group CHal Melting Point > 65 C OR Group CHal Molecular Weight > 280 g/mol OR Group CHal Molecular Weight > 370 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "r"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "s"

Referential boundary: The target chemical should be classified as Group CN log Kow > 4.5 by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "t"

Referential boundary: The target chemical should be classified as Not possible to classify according to these rules by Keratinocyte gene expression

Domain logical expression index: "u"

Referential boundary: The target chemical should be classified as High gene expression OR High gene expression >> Activated esters OR High gene expression >> alpha,beta-Carbonyl compounds with polarized double or triple bonds OR Low gene expression OR Low gene expression >> Long chain aldehydes OR Moderate gene expression OR Moderate gene expression >> Acrylate esters OR Moderate gene expression >> Aldehydes OR Moderate gene expression >> alpha,beta-Unsaturated aldehydes by Keratinocyte gene expression

Domain logical expression index: "v"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND (!Undefined)Group C Surface Tension > 62 mN/m by Skin irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "w"

Referential boundary: The target chemical should be classified as Group C Melting Point > 55 C by Skin irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "x"

Referential boundary: The target chemical should be classified as Alkene AND Allyl AND Cycloalkene AND Terpenes by Organic Functional groups ONLY

Domain logical expression index: "y"

Referential boundary: The target chemical should be classified as Alkene AND Allyl AND Cycloalkene AND Terpenes by Organic Functional groups ONLY

Domain logical expression index: "z"

Referential boundary: The target chemical should be classified as Alkene AND Allyl AND Cycloalkene AND Terpenes by Organic Functional groups ONLY

Domain logical expression index: "aa"

Similarity boundary:Target: CC(=C)C1CCC(C)=CC1
Threshold=20%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "ab"

Similarity boundary:Target: CC(=C)C1CCC(C)=CC1
Threshold=60%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "ac"

Similarity boundary:Target: CC(=C)C1CCC(C)=CC1
Threshold=80%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "ad"

Referential boundary: The target chemical should be classified as Aliphatic/Alicyclic hydrocarbons (Alpha 2u-globulin nephropathy) Rank C by Repeated dose (HESS)

Domain logical expression index: "ae"

Referential boundary: The target chemical should be classified as Valproic acid (Hepatotoxicity) Alert by Repeated dose (HESS)

Domain logical expression index: "af"

Referential boundary: The target chemical should be classified as Aliphatic/Alicyclic hydrocarbons (Alpha 2u-globulin nephropathy) Rank C by Repeated dose (HESS)

Domain logical expression index: "ag"

Referential boundary: The target chemical should be classified as Perhexiline (Hepatotoxicity) Alert by Repeated dose (HESS)

Domain logical expression index: "ah"

Referential boundary: The target chemical should be classified as Aliphatic/Alicyclic hydrocarbons (Alpha 2u-globulin nephropathy) Rank C by Repeated dose (HESS)

Domain logical expression index: "ai"

Referential boundary: The target chemical should be classified as Alpha olefin (Less susceptible) No Rank by Repeated dose (HESS)

Domain logical expression index: "aj"

Referential boundary: The target chemical should be classified as Aliphatic/Alicyclic hydrocarbons (Alpha 2u-globulin nephropathy) Rank C by Repeated dose (HESS)

Domain logical expression index: "ak"

Referential boundary: The target chemical should be classified as Allyl esters (Hepatotoxicity) Rank A by Repeated dose (HESS)

Domain logical expression index: "al"

Referential boundary: The target chemical should be classified as Aliphatic Carbon [CH] AND Aliphatic Carbon [-CH2-] AND Aliphatic Carbon [-CH3] AND Olefinic carbon [=CH- or =C<] AND Olefinic carbon [=CH2] AND Tertiary Carbon by Organic functional groups (US EPA) ONLY

Domain logical expression index: "am"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "an"

Referential boundary: The target chemical should be classified as Alkyl by Organic Functional groups (nested)

Domain logical expression index: "ao"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "ap"

Referential boundary: The target chemical should be classified as Alkene by Organic Functional groups (nested)

Domain logical expression index: "aq"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "ar"

Referential boundary: The target chemical should be classified as Bridged-ring carbocycles OR Carboxylic acid ester OR Conjugated system by Organic Functional groups (nested)

Domain logical expression index: "as"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "at"

Referential boundary: The target chemical should be classified as Silane by Organic Functional groups (nested)

Domain logical expression index: "au"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "av"

Referential boundary: The target chemical should be classified as Isopropyl OR Ketone by Organic Functional groups (nested)

Domain logical expression index: "aw"

Referential boundary: The target chemical should be classified as Terpenes by Organic Functional groups (nested)

Domain logical expression index: "ax"

Referential boundary: The target chemical should be classified as Epoxide OR Ether by Organic Functional groups (nested)

Domain logical expression index: "ay"

Referential boundary: The target chemical should be classified as No alert found by rtER Expert System ver.1 - USEPA

Domain logical expression index: "az"

Referential boundary: The target chemical should be classified as Multi Cyclic Hydrocarbons by rtER Expert System ver.1 - USEPA

Domain logical expression index: "ba"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "bb"

Referential boundary: The target chemical should be classified as Group C Aqueous Solubility < 0.0005 g/L by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "bc"

Referential boundary: The target chemical should be classified as -C=CH  [alkenyl hydrogen] AND -CH -  [cyclic] AND -CH2-  [cyclic] AND Methyl  [-CH3] by Bioaccumulation - metabolism alerts

Domain logical expression index: "bd"

Referential boundary: The target chemical should be classified as Linear C4 terminal chain  [CCC-CH3] by Bioaccumulation - metabolism alerts

Domain logical expression index: "be"

Referential boundary: The target chemical should be classified as Low (Class I) by Toxic hazard classification by Cramer (original) ONLY

Domain logical expression index: "bf"

Referential boundary: The target chemical should be classified as Does NOT Biodegrade Fast by Biodeg probability (Biowin 6) ONLY

Domain logical expression index: "bg"

Referential boundary: The target chemical should be classified as Not categorized by US-EPA New Chemical Categories

Domain logical expression index: "bh"

Referential boundary: The target chemical should be classified as Neutral Organics by US-EPA New Chemical Categories

Domain logical expression index: "bi"

Parametric boundary:The target chemical should have a value of log Kow which is >= 3.38

Domain logical expression index: "bj"

Parametric boundary:The target chemical should have a value of log Kow which is <= 5.68

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

Black pepper oil is considered to be irritating to rabbit eye.

Executive summary:

The ocular irritation potential of Black pepper oil was estimated using OECD QSAR toolbox v3.4 with logPow as the primary descriptor.

Black pepper oil was estimated to be irritating to the eye of New Zealand White rabbits.

Based on the estimated results, Black pepper can be considered to be irritating to eye and can be classified under the category “ Category 2 irritating to eye” as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin irritation:

In different studies, Black pepper oil has been investigated for potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits along with human data for target chemical Black pepper oil. The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

The dermal irritation potential of Black pepper oil was estimated using OECD QSAR toolbox v3.3 with logPow as the primary descriptor.

Black pepper oil was estimated to be not irritating to the skin of New Zealand White rabbits.Based on the estimated results, Black pepper can be considered to be not irritating to skin and can be classified under the category “Not Classified” as per CLP regulation.

In various studies summarized in Food and Cosmetics Toxicology. V.19, 1963 for the target Black pepper oil experimental results for its dermal irritation potential was provided for Mouse, Swine, Human and Rabbit.

Mouse:

 Black pepper oil was used as a test material to evaluate its skin irritation potential. Black pepper oil when applied on the back of hairless mice in undiluted for 24 hrs showed no skin reactions. Hence , Black pepper oil was considered to be not irritating to the skin of mice.

Swine:

Black pepper oil when applied on the back of hairless mice in undiluted form for 24 hrs showed no skin reactions. Hence , Black pepper oil was considered to be not irritating to the skin of swine

Human:

Black pepper oil was used as a test material to evaluate it’s skin irritation potential .

  Black pepper oil was applied on human subjects in a closed patch test in concentration 4 % in petrolatum. No positive skin reaction was observed. Hence, Black pepper oil was considered to be not irritating to human skin.

 Rabbit:

 Black pepper oil was used as a test material to evaluate its skin irritation potential on intact or abraded skin of rabbit . Black pepper oil was applied on the rabbit skin under occlusion for 24 hrs . Positive skin reactions were observed. Hence, Black pepper oil was considered to be moderately irritating to rabbit skin.

  Even though rabbit study indicates thatBlack pepper oilis moderately irritating to rabbit skin but Human , Swine, and mouse data concludes that it is not irritating to skin. Since, In the varied results of test animal maximum number of test animals produced no dermal irritation in response to black pepper oil . So,Black pepper oilcan be considered to be not irritating to skin.

 

Based on the available data for the target , it can be concluded that Black pepper oil was not irritating to skin ; and it can be classified under the category “Not classified ” as per CLP regulation.

Eye irritation:

In different studies, Black pepper oil has been investigated for potential for ocular irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits along with the predicted data for the target Black pepper oil and its closely related read across substances (3S)-3,7-dimethylocta-1,6-dien-3-ol (78-70-6) and 3,7,11-trimethyl-1,6,10-dodecatrien-3-ol (7212-44-4). The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

The ocular irritation potential of Black pepper oil was estimated using OECD QSAR toolbox v3.4 with logPow as the primary descriptor. Black pepper oil was estimated to be irritating to the eye of New Zealand White rabbits.

Based on the estimated results, Black pepper can be considered to be irritating to eye and can be classified under the category “Category 2 irritating to eye” as per CLP regulation.

The above prediction was supported by experimental result summarized in Hazardous Substances Data Bank (HSDB) for linalool U.S National Library of Medicine 2017 for the closely related read across substances (3S)-3,7-dimethylocta-1,6-dien-3-ol (78-70-6) (3S)-3,7-dimethylocta-1,6-dien-3-ol was used as test material to evaluate its eye irritation potential on 6 New Zealand white rabbits.

The test material was subjected to the conjunctive sac of rabbit in the concentration 0.1 ml (100%, 30% and 10%). Irritation was moderate at 100%, slightly at 30%; very slightly at 10%. As all the above concentrations produced positive ocular irritation reactions. Hence, (3S)-3,7-dimethylocta-1,6-dien-3-ol was considered to be eye irritant.

 The above experimental study was supported by experimental result summarized in Registry of Toxic Effects of Chemical Substances 2017 for the closely related read across substance (3S)-3,7-dimethylocta-1,6-dien-3-ol (78-70-6)

3,7,11-trimethyl-1,6,10-dodecatrien-3-ol was used as a test material on rabbits to evaluate its ocular irritation potential.3,7,11-trimethyl-1,6,10-dodecatrien-3-ol was subjected in the concentration 0.1ml , positive ocular reactions were observed . Hence, 3,7,11-trimethyl-1,6,10-dodecatrien-3-ol is considered to be mild eye irritant.

Based on the available data for the target and its closely related read across substances. it can be concluded that Black pepper oil was  irritating to eye ; and it can be classified under the category “Category 2 irritating to eye ” as per CLP regulation. 

Justification for classification or non-classification

Based on the available data for the target , it can be concluded that Black pepper oil was not irritating to skin ; and it can be classified under the category “Not classified ” as per CLP regulation. Whereas, based on the available data for the target and its closely related read across substances. it can be concluded that Black pepper oil was  irritating to eye ; and it can be classified under the category “Category 2 irritating to eye ” as per CLP regulation.