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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
19 Mar 1990
Reliability:
4 (not assignable)

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Qualifier:
according to guideline
Guideline:
other: Richtlinie 84/449/EWG (Teil C), EG Amtsblatt L 291/188
Qualifier:
according to guideline
Guideline:
other: DIN 38 409 Teil 3
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-Naphthalenesulfonic acid, 6-diazo-5,6-dihydro-5-oxo-, ester with phenyl(2,3,4-trihydroxyphenyl)methanone
EC Number:
270-931-7
EC Name:
1-Naphthalenesulfonic acid, 6-diazo-5,6-dihydro-5-oxo-, ester with phenyl(2,3,4-trihydroxyphenyl)methanone
Cas Number:
68510-93-0
Molecular formula:
all potential esters of C10HH5O3N2SCl and C13H10O4
IUPAC Name:
3-benzoyl-2-hydroxy-6-({[5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl 5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalene-1-sulfonate; 4-benzoyl-2,3-bis({[5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl 5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalene-1-sulfonate; 4-benzoyl-2,3-dihydroxyphenyl 5-oxo-6-(λ⁵-diazynylidene)-5,6-dihydronaphthalene-1-sulfonate
Test material form:
solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Sewage treatment plant Sindlingen, Frankfurt am Main, Germany (domestic)
- Preparation of inoculum for exposure: The pH of the activated sludge was adjusted from pH 5.8 to 7.6 using 1 M NaOH and then stirred for 24 h at 20 °C. The pH after stirring was 7.0.
- Initial cell/biomass concentration: 1 drop per Liter test solution.

Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
1 716 other: mg/g
Based on:
COD
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 20 °C
- pH: 7.0 (activated sludge)
- pH adjusted: The pH of the activated sludge was adjusted with 1 M NaOH.


TEST SYSTEM
- Test performed in open system: No


SAMPLING
- Sampling frequency: Oxygen measurements were performed after 7, 14, 21, and 28 d

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
ca. 39
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
not readily biodegradable
Conclusions:
Substance is not readily biodegradable.