4-methoxy-N-phenyl-o-toluidine

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates:

Toxicity to aquatic invertebrates of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated to be 13.39 mg/L when exposed to Daphnia magna for 48 hrs.

Short-term toxicity to aquatic invertebrates:

Toxicity to aquatic invertebrates of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated to be 13.39 mg/L when exposed to Daphnia magna for 48 hrs.

This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria

Additional information

Short-term toxicity to aquatic invertebrates:

Toxicity to aquatic invertebrates of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated to be 13.39 mg/L when exposed to Daphnia magna for 48 hrs.

This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria

The above prediction was supported by experimental result conducted by Sustainability Support Services (Europe) AB for the structurally similar read across substance Methyl 2-naphthyl ether (93-04-9).

Determination of the inhibition of the mobility of daphnids was carried out with the substance Methyl 2-naphthyl ether according to OECD Guideline 202.

The test substance was tested at the concentrations 0, 5, 10, 20, 30, 40, 80 mg/L and the immobilisation effects were observed for 48 hours

The median effective concentration (EC50) for the test substance, Methyl 2-naphthyl ether, in Daphnia magna was determined to be 26 mg/L for immobilisation effects.

This value indicates that the substance is likely to be hazardous to aquatic invertebrates can can be classified as Aquatic chronic category 3 as per the CLP criteria.

Another experimental data describing the toxicity to aquatic invertebrates  for the structurally similar read across substance 4 methoxyaniline (104-94-9) conducted by Renate Kuhn, Monika Pattard, Klaus-Dieter Pernak and Angela Winter summarized in Wat. Res. Vol. 23, No. 4, pp. 495-499, 1989.

Short term toxicity test was performed on daphnia magna for 24 hrs treated with p-methoxyaniline. The test vessel containing 52 mg l- 1of test material in 50ml beaker. No feed was providing during test period. The test vessel was observed for 24 and 48 hrs.So on the basis of above experiment it was found that the Effective concentration 50 value for daphnia magna was found to be 52 mg l- 1in 24 hrs after treatment with p-methoxyaniline (104-94-9).

This value indicates that the substance is likely to be hazardous to aquatic invertebrates can can be classified as Aquatic chronic category 3 as per the CLP criteria.

Based on the available data for the target and read across substances and applying the weight of evidence approach the target chemical 4-methoxy-2-methyl-N-phenylaniline can be classified as Aquatic chronic category 3 as per the CLP criteria.

Short-term toxicity to aquatic algae and cyanobacteria:

Toxicity to aquatic algae and cynobacteria of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated by using OECD QSAR tool box v3.3 using Log kow as primary descriptor and considering five closest read across substances. EC50 value of target chemical 4-methoxy-2-methyl-N-phenylaniline was estimated to be 11.24 mg/L when exposed to Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) for 72 hrs.

This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria.

The above prediction was supported by experimental data summarized in REGULATORY TOXICOLOGY AND PHARMACOLOGY 5, 123-131 (1985) for structurally similar read across substance 4 methoxyaniline (CAS 104-94-9).

The toxicity test was performed on algae for 4 methoxyaniline. The test was performed in analogy with OECD proposal (1979). The test material was analytically analysis by gas chromatography. The EC50 value for the subatance p-anisidine in algae (Scenedesmus pannonicus) was observed to be 14 mg/l.

This value indicates that the substance is likely to be hazardous to aquatic invertebrates can can be classified as Aquatic chronic category 3 as per the CLP criteria.

The above experimental data was further supported by experimental result summarized in Benzenamine, 4-Nitro-N-Phenyl - SIDS Initial Assessment Report , United Nations Environmental Programme (UNEP) 1998. For structurally similar read across substance 4-nitro-N-phenylaniline (CAS 836-30-6).

The acute toxicity test for algae was performed on Selenastrum capricornutum for the test chemical 4-nitro-N-phenylaniline , on the basis of cell growth inhibition after 96 hrs exposure period the EC50 value was observed as 27 mg/l.This value indicates that the substance is likely to be hazardous to aquatic invertebrates can can be classified as Aquatic chronic category 3 as per the CLP criteria.