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EC number: 247-063-2 | CAS number: 25513-64-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Nanomaterial radical formation potential
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Pseudomonas putida
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-02-23 to 1993-02-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part 8
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Stock solution: 6.25 g/l in aqua bidest.
- Nutrient solutions: According to guideline, no details reported
- Bacteria suspension: 436 nm extinction 0.156-0.160, adjusted to 0.150 with culture medium
- Addition of 80 ml stock solution (diluted at six levels) or aqua bidest., 2.5 ml nutrient solution II, 2.5 ml nutrient solution III, 5 ml nutrient solution
IV, 10 ml bacteria suspension or aqua bidest. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- TEST ORGANISMS
- Strain: Pseudomonas putida MIGULA - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- +/- 1 hour
- Test temperature:
- 20.7-21.3 °C
- pH:
- 7.2 (25 mg/l) - 8.6 (200 mg/l); not adjusted
- Nominal and measured concentrations:
- 25; 50; 75; 100; 150; 200 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Exposure vessel type: Sterile 250 ml Erlenmeyer flasks with cellulose plugs
- Number of replicates: 2 independent tests with each 3 replicates per exposure concentration, 5 growth controls (no test substance), 6 sterile
controls (no test organisms)
TEST PARAMETER
- light absorption at 436 nm, determined with Zeiss photometer PM 6: test solutions vs. sterile controls; growth controls vs. deionized water
STATISTICAL METHODS: Multiplan computer program "MIGULA" (regression analysis) - Reference substance (positive control):
- not specified
- Duration:
- 17 h
- Dose descriptor:
- EC10
- Effect conc.:
- 72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: mean value
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 89 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: mean value
- Details on results:
- Test 1: EC10 = 76.1 mg/l, EC50 = 92.0 mg/l
Test 2: EC10 = 67.5 mg/l, EC50 = 85.9 mg/l - Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 (16 hours) was determined as 89 mg/L indicating that the test substance may be harmful to aquatic microorganisms.
- Executive summary:
The effects of the test item on the bacteria species Pseudomonas putida were investigated by Hüls AG (1993) according to DIN 38412, part 8 (national German guideline). Six concentrations + control were tested over a period of 16 hours.
The EC50 was determined as 89 mg/L and the EC10 as 72 mg/L.
The study was assessed as "reliable without restrictions".
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1992-12-01 to 1992-12-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Principles of method if other than guideline:
- Method: other: DIN 38412 part 8
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Stock solution: 12.5 and 8.75 g/l in aqua bidest.
- Nutrient solutions: According to guideline, no details reported
- Bacteria suspension: 436 nm extinction 0.152-0.155, adjusted to 0.150 with culture medium
- Addition of 80 ml stock solution (diluted at six levels) or aqua bidest. 2.5 ml nutrient solution II; 2.5 ml nutrient solution III; 5 ml nutrient solution IV 10 ml bacteria suspension or aqua bidest. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- TEST ORGANISMS
- Strain: Pseudomonas putida MIGULA
- extinction at 436 nm: 0.155 and 0.152 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- +/- 1 hour
- Test temperature:
- 20.3-20.7 °C
- pH:
- Stock solution concentration pH before adjustment pH after adjustment
12.5 g/l 11.97 7.38
8.75 g/l 11.84 7.50 - Nominal and measured concentrations:
- 1750; 2500; 3500; 5000; 7000; 10000 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Renewal of test solution: no
- Exposure vessel type: Sterile 250 ml Erlenmeyer flasks with cellulose plugs
- Number of replicates: 2 independent tests with each 3 replicates per exposure concentration; 5 growth controls (no test substance); 6 sterile
controls (no test organisms)
TEST PARAMETER
- light absorption at 436 nm, determined with Zeiss photometer PM 6: test solutions vs. sterile controls; growth controls vs. deionized water
STATISTICAL METHODS: Multiplan computer program "MIGULA" (regression analysis) - Reference substance (positive control):
- no
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 469 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: mean value
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: mean value
- Details on results:
- Test 1: EC10 = 1697 mg/l, EC50 > 10 g/l
Test 2: EC10 = 1241 mg/l, EC50 > 10 g/l - Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 (16 hours) was determined as >10 g/L indicating that the test substance is not harmful to aquatic microorganisms.
- Executive summary:
The effects of the test item on the bacteria species Pseudomonas putida were investigated by Hüls AG (1993) according to DIN 38412, part 8 (national German guideline). Six concentrations + control were tested over a period of 16 hours. The pH of the stock solutions was adjusted to achieve a neutral value.
The EC50 was determined as >10 g/L and the EC10 as 1469 mg/L.
The study was assessed as "reliable without restrictions".
Referenceopen allclose all
Description of key information
The effects of the substance on the bacteria species Pseudomonas putida were investigated by Hüls AG (1993) according to DIN 38412, part 8 (national German guideline). Six concentrations + control were tested over a period of 16 hours. Two tests with and without pH adjustment were performed.
The EC50 for the test without pH adjustment was determined as 89 mg/L and the EC10 as 72 mg/L indicating that the test substance may be harmful to aquatic microorganisms.
Due to the basic properties of the test substance the concentrations used in the first test were rather low. Another test with higher concentrations and was conducted and the pH of the stock solutions was adjusted to achieve a neutral value. The EC50 was determined as >10 g/L and the EC10 as 1469 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 89 mg/L
- EC10 or NOEC for microorganisms:
- 72 mg/L
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