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Short-term toxicity to fish

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Description of key information

Acutely harmful to fish.

Key value for chemical safety assessment

Additional information

The key study concerning the short-term toxicity of 2,2'-dimethyl-4,4'-methylenebis(cyclohexylamin) to fish was performed according to OECD 203 (NITE Japan 2002) with Oryzias latipes. The 96-LC50 was determined to be 22.4 mg/L, the NOEC was 16 mg/L and the LC100 was 30 mg/L. These values are used for the assessment as Oryzias latipes was the most sensitive species to the substance.

The result is supported by a static-test study according to German industrial standard (DIN) 38412/15 (BASF 1988) performed with Leuciscus idus as test species. A 96-h LC50 of 31.6 mg/l was determined for non-neutralized samples (expressed as geometrical mean). The NOEC was determined to be 21.5 mg/l and the LC100 46.4 mg/l. Because DMD changes the pH in water, a neutralized sample with the highest test concentration (100 mg/l) was tested in addition. For this neutralized sample a diminution of the toxic effects was observed.

In addition, the result of the key study is supported by an acute toxicity test according to OECD 203, which was performed with Oryzas latipes in preparation of a bioaccumulation study (see also chapter 5.3.1). The LC50 after 96 hours was determined to be 32 mg/L (NITE Japan, 2002).