Registration Dossier

Toxicological information

Immunotoxicity

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Administrative data

Endpoint:
immunotoxicity: acute oral
Adequacy of study:
disregarded due to major methodological deficiencies

Data source

Reference
Reference Type:
publication
Title:
No information
Author:
GAWORSKI CL, VOLLMUTH TA, DOZIER MM, HECK JD, DUNN LT, RATAJCZAK HV, THOMAS PT
Year:
1994
Bibliographic source:
Food Chem Toxicol, 32(5), 409-415

Materials and methods

Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Vanillin
EC Number:
204-465-2
EC Name:
Vanillin
Cas Number:
121-33-5
Molecular formula:
C8H8O3
IUPAC Name:
4-hydroxy-3-methoxybenzaldehyde
Details on test material:
no data

Test animals

Species:
mouse
Strain:
CD-1
Sex:
female
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: methylcellulose
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
5 days
Frequency of treatment:
daily
Doses / concentrations
Remarks:
Doses / Concentrations:
250, 500 and 1000 mg/kg/day
Basis:
no data
No. of animals per sex per dose:
no data
Control animals:
yes, concurrent no treatment
Details on study design:
positive control group: host resistance to Listeria monocytogenes

Results and discussion

Results of examinations

Details on results:
In female CD-1 mice (30 mice/group) dosed intragastrically at 3 concentrations: 1000, 500, and 250 mg/kg/day Vanillin, and vehicle control
(1% methylcellulose) for 5 consecutive days; control untreated group, positive control group: host resistance to Listeria monocytogenes;
Anti-sheep red blood cell (SRBC) plaque-forming cell (PFC) assay: specific cellular activity, and total speen activity;
no significant difference in treated group by Vanillin from untreated control group.

Applicant's summary and conclusion