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EC number: 240-957-3 | CAS number: 16909-22-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29-06-2016 to 27-07-2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tetraethylammonium benzoate
- EC Number:
- 240-957-3
- EC Name:
- Tetraethylammonium benzoate
- Cas Number:
- 16909-22-1
- Molecular formula:
- C8H20N.C7H5O2
- IUPAC Name:
- tetraethylazanium benzoate
- Test material form:
- liquid
- Details on test material:
- tetraethylammonium benzoate manufactured by Evonik Degussa GmbH, batch BRA 1434, purity 49.9 w/w (excluding solvent)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Test system: Inoculum of the aqueous phase of non-adapted activated sludge.
Source: Municipal sewage treatment plant, 31137 Hildesheim, Germany
Reasons for the selection of the test system : Activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
Receipt: 2016-06-23
Pretreatment: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was re-suspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2 hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2-free air for 6 days. 10 mL/L were used to initiate inoculation.
Colony forming units in the test vessels: Approx. 10E7 - 10E8 CFU/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 52.1 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test Item
ThOD: 1.24 mg O2/mg
ThODNO3: 1.37 mg O2/mg (with nitrification)
Test concentration: 42.0 mg/L
ThOD in the test vessel: 52.1 mg O2/L (57.5 mg O2/L with nitrification)
Pretreatment: None
Inoculum Control: Test medium without test and / or reference item
Toxicity Control: Test and reference item in test concentration
Test Method
Duration: 28 days
Application: Once at test start
Test vessels: Brown glass bottles (volume 500 mL)
Test volume: 250 mL
Test medium: Mineral salts medium according to OECD 301 F
Temperature: Nominal: 20 - 24, ± 1 °C; Actual: 20.2 – 20.5 °C
Dispersion treatment: Continuous stirring
Photoperiod: Dark, in an incubator
Preaparation of the test vessels: Based on the calculated oxygen demand, the test concentration of 42 mg/L, corresponding to an oxygen demand of 52.1 mg O2/L (57.5 mg O2/L with nitrification) in the vessel, was selected.
The test solutions were prepared in measuring flasks and were placed in brown glass bottles as incubation vessels (inoculum control, functional control, test item and toxicity control):
• two for the inoculum control (C1, C2)
• one for the functional control (R1)
• two for the test item (P1, P2)
• one for the toxicity control (T1)
The inoculated test medium, consisting of the required volumes of mineral medium stock solutions, ultrapure water and inoculum, was prepared in a measuring flask. 250 mL of this solution were filled in the brown glass bottles of the inoculum control, using a 250 mL measuring flask.
For the functional control, the reference item was weighed out and transferred into a measuring flask, the required vol-umes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution were filled in the brown glass bottle of the functional control, using a 250 mL measuring flask.
For the test item replicates, the test item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution were filled in the brown glass bottles of the test item replicates, using a 250 mL measuring flask.
For the toxicity control, the test and reference item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution were filled in the brown glass bottle of the toxicity control, using a 250 mL measuring flask.
A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2. The bottles were closed with OxiTop measuring heads and the measuring system was activated.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- In the toxicity control containing both test and reference item 67% degradation occurred within 14 days. After 28 days the biodegradation came to 74%. The degradation of the reference item was not inhibited by the test item.
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 36
- Sampling time:
- 28 d
- Details on results:
- pH-Values at Test Start and Test End
The pH-values of the samples were measured at test start and test end (day 28).
pH-Value
Start End
Inoculum Control 7.70 1) 7.70
2) 7.67
Functional Control 7.71 7.86
Test Item 7.73 1) 7.68
2) 7.70
Toxicity Control 7.71 7.94
1), 2) = First, second replicate
BOD5 / COD results
- Results with reference substance:
- In order to check the activity of the test system sodium benzoate was used as functional control. The pass level > 60% was reached on day 4. The biodegradation reached a max-imum of 93 % degradation on day 24.
Any other information on results incl. tables
Biodegradation [%] of the Test Item inComparison to the Functional Control and Toxicity Control
|
Biodegradation [%] |
||||
|
Study Day [d] |
||||
|
Replicate |
7 |
14 |
21 |
28 |
Test Item |
1 |
37 |
41 |
39 |
39 |
2 |
34 |
36 |
35 |
33 |
|
Functional Control |
|
80 |
89 |
92 |
92 |
Toxicity Control |
|
61 |
67 |
70 |
74 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item is classified as not readily biodegradable within the 28 day period of the study.
- Executive summary:
The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days. The study was conducted from 2016-06-29 to 2016-07-27 according to OECD guideline 301 F and guideline EC C.4-D atthe test facility. The test item concentration selected as appropriate was 42 mg/L, corresponding to a ThOD of 52.1 mg O2/L per test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day.
The mean oxygen depletion in the inoculum control was 11.2 mg O2/L on day 28.
In order to check the activity of the test system sodium benzoate was used as functional control. The pass level > 60% was reached on day 4. The biodegradation reached a maximum of 93 % degradation on day 24.
In the toxicity control containing both test and reference item 67% degradation occurred within 14 days. After 28 days the biodegradation came to 74%. The degradation of the reference item was not inhibited by the test item.
The biodegradation of the test item is in comparison to the readily degradable functional control and the toxicity control. Both test item replicates reached the 10% level (beginning of biodegradation) on day 2. The 60% pass level was not reached until test end and the mean biodegradation on day 28 was 36%.
The calculations were performed with the ThOD instead of the ThODNO3, because no indication for the typical biphasic course of biodegradation was observed and the recorded oxygen consumption resembles the oxygen demand of the benzoate moiety of the test item. Benzoate functions also as the readily biodegradable functional control and is therefore known to be easily degraded, while tertiary amines are known to be stable towards hydrolysis. Therefore, it is assumed that only the benzoate from the test item was degraded and no nitrification occurred.
The validity criteria of the guideline are fulfilled.
Conclusion
The test item is classified as not readily biodegradable within the 28 day period of the study.
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