Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-957-3 | CAS number: 16909-22-1
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
In the acute immobilization test to Daphnia magna (STRAUS), the effects of the limit concentration of 200 mg/L of the test item, which corresponds to 100 mg/L of the main component, were determined at the test facility from 2016‑12‑13 to 2016-12-15 according to OECD 202 (2004), which is equivalent to the Council Regulation (EC) No.440/2008 Method C.2 (2008).
The study was conducted under static conditions over a period of 48 hours.The limit concentration (200 mg/Lof the test item were weighed out) was freshly prepared with dilution water before the start of the exposure (0 hours).The limit concentration was mixed thoroughly by manual agitation until the test item was completely dissolved. The limit concentration was visually clear during the exposure period.
Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the limit concentration and the control.
The concentration of the main component was analytically verified by LC-MS/MS at the start (0 hours) and at the end of exposure (48 hours) at the limit concentration and the control.
The measured concentration of test item at the start of the exposure (0 hours) was 91% of the nominal value at the limit concentration level. The measured concentrations at the end of the exposure (48 hours) was 105% of the nominal value.
The measured concentrations of test item were within ± 20% of the nominal concentration. This indicates that the test item concentration was successfully maintained for the duration of the test. Therefore, all effect concentrations given are based on the nominal concentration of the test item. Additionally, the effect concentrations are given based on the nominal concentration of the main component.
The validity criteria of the test guideline were fulfilled.
Conclusion
At the limit concentration of 200 mg/L of the test item, which corresponds to a nominal concentration of 100 mg/L of the main component, no effects on Daphnia magna were observed.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 200 mg/L
Additional information
At the limit concentration of 200 mg/L of the test item, which corresponds to a nominal concentration of 100 mg/L of the main component, no effects on Daphnia magna were observed.
EC50 > 200 mg/L (test item)
EC50 > 100 mg/L (main component)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
