Reaction mass of 1,4-bis(methylamino)anthraquinone and 1,4-bis[(2-ethylhexyl)amino]anthraquinone and 1-[(2-ethylhexyl)amino]-4-(methylamino)anthraquinone and 9,10-Anthracenedione, 1,4-bis(pentylamino)-, branched and linear and 9,10-Anthracenedione, 1-(methylamino)-4-(pentylamino)-, branched and linear and 9,10-Anthracenedione, 1-[(2-ethylhexyl)amino]-4-(pentylamino)-, branched and linear

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is highly insoluble in water, hence indicating that aquatic toxicity is unlikely to occur

Justification for type of information:

According to QSAR estimation methods for the three known components of C.I. Solvent Blue 98 (3 Amine), the water solubility ranged anywhere from 4.0E-008 to 1.2 mg/L. Using the flask method, the water solubility of C.I. Solvent Blue 98 (3 Amine) was empirically determined to be 0.319 mg/L at 20°C. Using the HPLC method, the n-octanol/water partition coefficient was determined to range from 1.0E006 to greater than 3.2E006 (i.e. log Kow of 6.01 to >6.5). This was also similar to the Log Kow estimates for the known components of C.I. Solvent 98 (3 amine) according to KOWWIN (v. 1.43) which predicted log Kow values ranging from 4.2 to 11.0.

Using the ECOSAR program (developed by the US Environmental Protection Agency’s Office of Chemical Safety and Pollution Prevention, v 1.11), the predicted effect levels for both acute and chronic endpoints with aquatic algae exceeded the estimated water solubility by more than 10X for the chemical structures evaluated. Thus, no toxicity effects toward microorganisms at water saturation were predicted for the test material, and the study was deemed scientifically not necessary.

No acute or chronic toxicity effects at water saturation were predicted for the test material, therefore toxicity testing with microorganisms is waived since the study is scientifically not necessary.

Description of key information

According to QSAR estimation methods for the three known components of C.I. Solvent Blue 98 (3 Amine), the water solubility ranged anywhere from 4.0E-008 to 1.2 mg/L. Using the flask method, the water solubility of C.I. Solvent Blue 98 (3 Amine) was empirically determined to be 0.319 mg/L at 20°C. Using the HPLC method, then-octanol/water partition coefficient was determined to range from 1.0E006 to greater than 3.2E006 (i.e. log Kow of 6.01 to >6.5). This was also similar to the Log Kow estimates for the known components of C.I. Solvent 98 (3 amine) according to KOWWIN (v. 1.43) which predicted log Kow values ranging from 4.2 to 11.0.

Using the ECOSAR program (developed by the US Environmental Protection Agency’s Office of Chemical Safety and Pollution Prevention, v 1.11), the predicted effect levels for both acute and chronic endpoints with aquatic algae exceeded the estimated water solubility by more than 10X for the chemical structures evaluated. Thus, no toxicity effects toward microorganisms at water saturation were predicted for the test material, and the study was deemed scientifically not necessary.

No acute or chronic toxicity effects at water saturation were predicted for the test material, therefore toxicity testing with microorganisms is waived since the study is scientifically not necessary.