Reaction product of 2-Propenoic acid and Oxirane, mono[(C12-16-alkyloxy)methyl] derivs.

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 Sep - 21 Oct 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

Jul 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Würtemberg (29 Jun 2016).

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Municipal wastewater treatment plant, Breisgauer Bucht, Forchheim, Germany
- Storage length: The sludge was sampled on 20 Sep 2016 and the test was initiated on 22 Sep 2016.
- Preparation of inoculum for exposure: The activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge.
- Concentration of sludge: The dry solid content of the sampled activated sludge was 3.6 g/L (mean of triplicate weight measurements after 3 h drying at 105 °C).
- Initial sludge concentration: 30 mg dry solids/L

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Initial conc.:

>= 129.1 - <= 132.1 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Mineral medium
- Test temperature: 21.9 - 22.4 °C
- pH: 7.6 (initial pH), 7.6 (final pH on Day 28 in test vessels), and 7.5 (in blank vessels)
- pH adjusted: No
- Aeration of dilution water: The mineral medium was aerated for about 1 h before use.
- Suspended solids concentration: 30 mg dry solids/L

TEST SYSTEM
- Culturing apparatus: Oxitop-System from WTW, Weilheim consisting of narrow-necked glass bottles with rubber sleeve inserts for the CO2 trap for the absorption of the evolved CO2. Magnetic stirrers are introduced into the bottles, which are placed on a stirrer platform.
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Aeration of mineral medium before use.
- Measuring equipment: Oxitop measuring heads with a measuring range of 500 - 1350 hPa.
- Details of trap for CO2 and volatile organics if used: Rubber sleeve inserts with NaOH pellets.
- Other: The liquid volume was fixed to 164 mL.

CONTROL AND BLANK SYSTEM
- Inoculum blank: Three reactors containing only inoculum.
- Toxicity control: One reactor containing the test item (232.1 mg/L) and reference compound.
- Procedure control: Three reactors containing the reference compound.

Reference substance:

acetic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Value:

77.5

Sampling time:

28 d

Details on results:

Degradation was > 60% on Day 10.

TOXICITY CONTROL
The test item had no toxic effect on the inoculum (42.2% degradation within 4 d) according to the validity criteria of OECD 301 (< 25% on Day 14). The pH in the vessel was 8.6.

Results with reference substance:

The reference substance (sodium acetate) reached the pass level for ready biodegradability (≥ 60% ThOD and fulfilment of the 10-d window) within 8 d.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

77.8% was degraded within the 28 days (O2-consumption). The substance is concluded to be readily biodegradable.

Executive summary:

77.8% was degraded within the 28 days (O2-consumption). The substance is concluded to be readily biodegradable.

Description of key information

77.8% was degraded within the 28 days (O2-consumption). The substance is concluded to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater