Reaction product of 2-Propenoic acid and Oxirane, mono[(C12-16-alkyloxy)methyl] derivs.

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019.08.22 - 2019.12.19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was performed in accordance with the OECD Principles of Good Laboratory Practice as accepted by Regulatory Authorities throughout the European Union, United States of America (FDA and EPA), Japan (MHLW, MAFF and METI) and other countries that are signatories to the OECD Mutual Acceptance of Data Agreement.

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

OECD Guideline 209. "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)”, adopted 22 July 2010.

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification: Reaction product of 2-Propenoic acid and Oxirane, mono[(C12-16-alkyloxy)methyl] derivs. Batch (Lot) Number: 190708588, Expiry date: 31 July 2020, Physical Description: Transparent liquid, Purity/Composition: See Certificate of Analysis, UVCB, Storage Conditions: At room temperature protected from light

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

The sludge was taken from the municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
The sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. The concentration of the suspended solids was determined (3 g/L, as used for the test). The batch of sludge was used one day after collection. Therefore 50 mL of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use. The initial pH of the sludge was 5.6 on the day of testing. The sludge was buffered to a pH of 7.0 using a 70 g/L sodium bicarbonate solution. Before the sludge was used, the respiration rate of the sludge was determined to verify the quality of the sludge. The mean respiration rate was 23 mg O2/g sludge and was within the validity criterion.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Hardness:

CaCl2.2H2O 211.5 mg/L

Test temperature:

The temperature continuously measured in the temperature control vessels ranged between 19.4 and 20.7°C during the test, and complied with the requirements as laid down in the Study Plan (20 ± 2°C).

pH:

The pH in all test vessels, before addition of sludge, was between 7.4 and 7.7. After the 3 hour exposure period the pH was between 7.3 and 8.0.

Nominal and measured concentrations:

Test concentration(s) 20, 50, 140, 400 and 1000 mg/L (nominal)

Reference substance (positive control):

yes

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

96 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

other: Confidence interval 14.2 – 190.6 mg/L

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/L for total respiration (3.9 mg/L in the final test)

Reported statistics and error estimates:

NOEC 50 mg/L
EC10 96.2 (14.2 – 190.6 mg/L)
EC20 300 (130.4 – 498.8 mg/L)
EC50 >1000 mg/L

Validity criteria fulfilled:

yes

Conclusions:

NOEC 50 mg/L
EC10 96.2 (14.2 – 190.6 mg/L)
EC20 300 (130.4 – 498.8 mg/L)
EC50 >1000 mg/L

Executive summary:

The conditions of the present test the test item “Reaction product of 2 -Propenoic acid and Oxirane, mono[(C12-16-alkyloxy)methyl] derivs” was not toxic to waste water bacteria (activated sludge) at or below a concentration of 50 mg/L (NOEC).

The EC10 was 96.2 mg/L (95% confidence interval: 14.2 - 191 mg/L).

The EC20 was 300 mg/L (95% confidence interval: 130 - 499 mg/L).

The EC50 was >1000 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

EC10 or NOEC for microorganisms:

96.2 mg/L