Reaction mass of 1-Propanaminium, 2,3-dihydroxy-N,N-dimethyl-N-[3-[(1-oxododecyl)amino]propyl]-, chloride and 1-Propanaminium, 2,3-dihydroxy-N,N-dimethyl-N-[3-[(1-oxotetradecyl)amino]propyl]- , chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

december 29, 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Title:

Unnamed

Year:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

40 CFR Part 797.1300 and 50 FR 39321, 9/27/85 "Daphnid acute toxicity test"

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cocamidopropyl PG-dimonium chloride

Cas Number:

136920-08-6

Molecular formula:

C20-30H43-63ClN2O2 range

IUPAC Name:

Cocamidopropyl PG-dimonium chloride

Test material form:

other: aqueous solution

Details on test material:

Lot# HH105P
Cocamidopropyl PG dimonium chloride
Lexquat AMG-WC

Specific details on test material used for the study:

sample storage in ambient laboratory 21C, sealed product container

Sampling and analysis

Analytical monitoring:

yes

Remarks:

US EPA (NPDES) Probit Analysis Version 1.2

Test solutions

Details on test solutions:

Exposure volume 15 mL
Replication: 5 daphnids/replicate, 4 replicates/treatment
Concentrations: 0.10, 0.18, 0.32, 0.56, and 1.0 ppm nominal

Test organisms

Test organisms (species):

Daphnia pulex

Details on test organisms:

organism age < 24 hrs at test initiation
organism history: USTC 57th generation, 5th brood, 6/19/89
Feeding regime: none during test

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Round valley reservoir, Lebanon, NJ activated carbon stripped and 0.45micron filtered

Total exposure duration:

48 h

Test conditions

Hardness:

120 mg/L CaCO3

Test temperature:

20 +/- 2 C

pH:

pH (24hrs) = 8.3
pH (48hrs) = 7.8

Dissolved oxygen:

9.0 mg/L at both 24 hr and 48 hr test

Salinity:

alkalinity: 50 mg/L CaCO3

Conductivity:

At 24hr test: 110 microm hos
At 48hr test: 120 microm hos

Nominal and measured concentrations:

nominal concentrations: 0.10 / 0.18 / 0.32 / 0.56 / 1.0 ppm

Details on test conditions:

Aeration: aerate by mixing test solutions to saturation prior to test; no aeration during test required.
test vessels: 18 x 150 mm glass tubes with Kaput caps
Illumination: 16:8 hour light/dark cycle fluorescent, 50 to 100 ft-candles (lab ambient)

Reference substance (positive control):

yes

Remarks:

diluent, no solvent required

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.6 other: ppm

Nominal / measured:

nominal

Basis for effect:

mortality

Details on results:

The LC50 (48hrs) = 0.60 ppm obtained represents toxicity caused solely by the product. Control mortality was absent and a consistent trend of increasing mortality with increasing product concentration was observed.
Adsorption of the product to exposure chambers may have occured during the test. However daily renewal of test concentrations mitigated these effects, if ony partially.
Chemical confirmation of nominal concentrations was not achieved however the LC50 estimate, are considered reasonably accurate and representative of the type of moiety.

Results with reference substance (positive control):

No moratlity after 48hr test

Reported statistics and error estimates:

HPLC determination of nominal, low, high, and control concentrations was attempted. Frozen, coded samples were sent to the Sponsor July 12, 1989, but were not analysed until October 1989. In addition, the results were questionable since:
1) controls had detectable levels of test substance that should not in theory be present
2) analysed amount of products in test concentrations samples were from one to three orders of magnitude different (lower) from the nominal concentrations intended wuthou any obvious trend, or the levels were undetectable,
3) the methods could not distinguish among the three componds tested and may not be accurate at the low levels tested. Hence the chemical confirmation method may be flawed or the sample storage may have affected the results of chemical analysis or both.

Applicant's summary and conclusion

Conclusions:

48 hr LC50= 0.60 ppm nominal concentration (95% confidence limits = 0.51 - 0.72)

Executive summary:

Using 40 CFR Part 797.1300 and 50 FR 39321, 9/27/85 "Daphnid acute toxicity test", the toxicity to aquatic invertebrates (Daphnia Pulex) has been described by nominal concentration in a 48 hr test. The result is:

48 hr LC50= 0.60 ppm nominal concentration (95% confidence limits = 0.51 - 0.72)