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EC number: 232-667-0 | CAS number: 9003-98-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 19 June 1990 - 4 January 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- This study is used as cross reference. Due to the similarity between enzymes, the same conclusion can be reached for deoxyribonuclease.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Active enzyme protein of Lipase, triacylglycerol (EC no. 232-619-9, CAS no. 9001-62-1, EC name: Lipase, triacylglycerol, Enzyme Class No.: 3.1.1.3)
- Cas Number:
- 9001-62-1
- Molecular formula:
- Not applicable, see remarks
- IUPAC Name:
- Active enzyme protein of Lipase, triacylglycerol (EC no. 232-619-9, CAS no. 9001-62-1, EC name: Lipase, triacylglycerol, Enzyme Class No.: 3.1.1.3)
- Reference substance name:
- Protein as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available
- IUPAC Name:
- Protein as a constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Carbohydrates constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Carbohydrates constituent of enzyme deriving from the fermentation or extraction process
- Reference substance name:
- Lipids as a constituent of enzyme deriving from the fermentation or extraction process
- Molecular formula:
- Not available. See remarks.
- IUPAC Name:
- Lipids as a constituent of enzyme deriving from the fermentation or extraction process
- Test material form:
- liquid
- Details on test material:
- - Lot/batch No.: LAJR 009-12
- Expiration date of the lot/batch: 1 August 1998
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Constituent 5
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: The aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage.
- Preparation of inoculum for exposure: The aerated sample was filtered through a coarse filter paper (first approximately 200 mL discarded) and used on day of collection.
- Concentration of sludge: 0.5 mL of inoculum per litre dilution water - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 250 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 40 mg/L
- Based on:
- ThIC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition oftest medium: 1000 mL dilution water plus 1 mL nutrient solutions (a)-(f): (a) potassium dihydrogen phosphate: 8.5 g/L; di-potassium hydrogen phosphate: 21.75 g/L; di-sodium monohydrogen phosphate dihydrate: 33.4 g/L; ammonium chloride: 20 g/L), (b) magnesium sulphate heptahydrate (22.5 g/L), (c) calcium chloride (27.5 g/L), (d) iron(III)chloride hexahydrate (0.25 g/L), (e) Mangan sulphate tetrahydrate (39.9 mg/L), boric acid (57.2 mg/L), zinc sulphate heptahydrate (42.8 mg/L), ammonium molybdate tetrahydrate (36.85 mg/L) and (f) Yeast extract 15 mg/100 mL; plus 0.5 mL inoculum plus sufficient test substance. This is diluted to 2 litres with dilution water.
- Test temperature: 22 ± 1°C
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2 controls, 2 test substance and 2 reference substance
- Method used to create aerobic conditions: Aeration by magnetic stirrers and compressed air via narrow bore glass tubes.
- Measuring equipment: IONICS TC/TOC Analyser model 555.
- Test performed in open system: yes
SAMPLING
- Sampling frequency: day 0, 1,3, 7, 14, 21, 27 and 28
- Sampling method: Samples (ca. 20 mL) were withdrawn from each vessel and filtered through Sartorius Minisart NML, 0.45 µm disposable filters, discarding the first 10 mL.
- Sample storage before analysis: Samples were analysed directly
CONTROL AND BLANK SYSTEM
- Inoculum blank: inoculated test medium
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 44
- Sampling time:
- 1 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 79
- Sampling time:
- 3 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 92
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate achieved 79 % degradation within 3 days and 95% degradation within 28 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the conditions of the test Lipase SP 400 was found to be readily biodegradable.
- Executive summary:
Lipase SP 400 was assessed for ready biodegradability according to OECD Guideline 301E and EEC Directive 67/548 Annex V C.3 as published in 84/449/EEC, and in compliance with GLP.
SP 400 achieved 79% biodegradation within 3 days and 99% biodegradation within 28 days. The pass level of 70% was thus reached within 10 days of exceeding the 10% level.
The reference substance attained 95% biodegradation within 28 days, thereby confirmiung the suitability of the inoculum and the culture conditions.
In conclusion, SP 400 was found to be readily biodegradable under the conditions of the test.
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