Paraffin oils, sulfochlorinated, saponified

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-03-11 till 1991-04-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

SAMPLE COLLECTION
The samples were collected by staff members of the ecotoxicology group. The samples were transferred into amber hottles and stored until analysis at below -20°C.

HANDLING
Analyses were performed in duplicate, no sample was stored.

Vehicle:

no

Details on test solutions:

- Date of purchase: March 26, 1991
- Size of stock vessel: 182 L; height: 49 cm; length: 98 cm; with: 38 cm Material of stock vessel: glass
- Amount of water per stock vessel: 149 L
- Depth of water in the stock vessel: approximately 40 cm
- Estimated number of fish kept as a stock per holding vessel (batchsize): .200
- Water: reconstituted water
- Renewal of water: Flow through - weekly turnover rate: approximately 1000-1500 L Temperature of water: 20/22 min/max °C
- Oxygen content of water: 80/100 min/max % 8.5/9.5 min/max mg/L
- Hardness of water: 150/250 min/max mg CaCO3/L
Under holding conditions the hardness of water is measured infrequently. The lowest and highest value ever measured is given above. However, themore realistic values correspond to those measured for the reconstituted water in this study since the reconstituted water for the holding of fish
and the test were derived from the same source.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Test Species: Brachydanio rerio (Hamilton Buchanan), Zebra fish
- Supplier: Zoozentrum Höchst, Frankfurt a.M.
- Starting Date (in-life phase): 1991-03-11
- Completion Date (in-life phase): 1991-04-13
- Average body weight of fish (sample consisting of 10 fish): 0.209 ± 0.027 (S.D.) g/fish Loading: 0.281 g/L

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

8.4 - 14.0°dH (150 - 250 mg CaCO3/L)

Test temperature:

20 -22 °C

pH:

7.5 - 7.9

Dissolved oxygen:

Oxygen content of water: 8.5/9.5 mg/L (80/100 %)

Nominal and measured concentrations:

Nominal concentration of the stock solution: 101.5 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Test concentration was analytically confirmed

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Test concentration was analytically confirmed

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Test concentration was analytically confirmed

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.66 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Based on measured concentration at the end of the test

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Based on measured concentration at the end of the test

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.65 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Based on measured concentration at the end of the test

Details on results:

- The analytically determined actual concentrations of the test material in the test solutions at the beginning of the study were found to be within the range of 109 % and 143 % with an average of 121.3 %.
- The analytically determined stability of the test material in the test solutions after 96 hours of exposure was found to be within the range of 13 % and 42 % of the nominal values with an average of 26.5 %.

Reported statistics and error estimates:

Probit Analysis (Finney 1971) was used to determine LC-values. When less than 3 test substance concentrations caused effects between O and 100 % then the geometrical mean was applied.

Sublethal observations / clinical signs:

MBAS Content of the Product

Nominal Amount of Emulgator E 30 fest	Extinction E650	µg MBAS determined
2000 µg	n.d.	n.d.
1000 µg	3.5	682.1
500 µg	1.025	199.8
200 µg	0.661	128.8

n.d. = not determinable because the values were out of range

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of Paraffin oils, sulfochlorinated, saponified to Fish (Danio rerio) was tested according to OECD Guideline no. 203 "Fish, Acute Toxicity Test, adopted 1984". The lethal concetration LC50 was determined during 96 hours to be 1.10 mg/L (Leuna, AG, 1991).

Executive summary:

In accordance with GLP regulations the acute toxicity of Emulgator E 30 to the fish Brachydanio rerio (Hamilton Buchanan), Zebra fish was investigated in a static test system during a test period of 96 hours. The study was performed according to the OECD Guideline 203, 1984.

The nominal concentrations to which the test organisms were exposed were 0.625, 1.25, 2.5, 5.0 and 10.0 mg/L Emulgator E 30.

The analytically determined actual concentrations of the test material in the test solutions at the beginning of the study were found to be within the range of 109% and 143% with an average of 121.3%.

Based on nominal concentrations the study resulted in the following value:

LC50 (96 h): 4.16 mg/L Emulgator E 30

No Observed Effect Concentration (NOEC): 2.50 mg/L Emulgator E 30

Based on actual (measured) concentrations at the end of the test ( on average 26.5% nominal) the study resulted in the following value:

LC50 (96 h): 1.10 mg/L Emulgator E 30

No Observed Effect Concentration (NOEC): 0.66 mg/L Emulgator E 30

Description of key information

The acute toxicity of Paraffin oils, sulfochlorinated, saponified to Fish (Danio rerio) was tested according to OECD Guideline no. 203 "Fish, Acute Toxicity Test, adopted 1984". The lethal concentration LC50 was determined during 96 hours to be 1.10 mg/L (Leuna, AG 1991).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.1 mg/L

Additional information

Result based on actual (measured) concentrations at the end of the test (on average 26.5% nominal)