1,2,3-Propanetricarboxylic acid, 2-hydroxy-, chromium(3+) salt (1:1)

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Guideline study to GLP
Confirms irritancy of simple organic acid salts of chromium III

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Chromium acetate

EC Number:

241-562-9

EC Name:

Chromium acetate

Cas Number:

17593-70-3

Molecular formula:

3Cr, < 3(C2H4O2) < 1(OH)

IUPAC Name:

Chromium III hydroxyacetate

Results and discussion

In vitro

Results

Irritation parameter:

in vitro irritation score

Run / experiment:

1

Value:

ca. 4.4

Vehicle controls validity:

valid

Negative controls validity:

valid

Positive controls validity:

valid

Other effects / acceptance of results:

The low viability is close to figures seen for Eye Dam 1.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

It is noted that this method alone cannot validate the division between Eye Dam 1 and Eye Irrit 2, but acetates are weak acids and although the class if substance will show significant effect in this in-vitro assay, impact on the cornea (ie Eye Dam 1) is not expected.
Citric acid is a weaker acid than acetic and this result suggests that Eye Irrit 2 is a valid classification.