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EC number: 231-836-6 | CAS number: 7758-19-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- genetic toxicity in vivo, other
- Remarks:
- Type of genotoxicity: other: Sperm-head anomalies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Test method was not according to any guideline. No data on GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of Chemicals Used for Drinking Water Disinfection for Production of Chromosomal Damage and Sperm-Head Abnormalities in Mice
- Author:
- Meier JR, Bull RJ, Stober JA, Cimino MC
- Year:
- 1 985
- Bibliographic source:
- Environmental Mutagenesis 7:201-211.
Materials and methods
- Principles of method if other than guideline:
- Male B6C3F1 mice (10 per group) were administered total doses of 0.2, 0.5 or 1 mg sodium chlorite by gavage in five equal daily doses. Positive controls received 200 mg ethyl methanesulfonate/kg bw intraperitoneally in five equal doses. Animals were sacrificed 1, 3 and 5 weeks after dosing, and the caudae epididymides were removed and diced in saline. Tissue fragments were filtered out, and 1000 sperm heads were scored per animal for abnormalities.
- GLP compliance:
- not specified
- Type of assay:
- other: Sperm-head anomalies
Test material
- Reference substance name:
- Sodium chlorite
- EC Number:
- 231-836-6
- EC Name:
- Sodium chlorite
- Cas Number:
- 7758-19-2
- Molecular formula:
- ClHO2.Na
- IUPAC Name:
- sodium chlorite
- Details on test material:
- - Name of test material (as cited in study report): Sodium chlorite, NaClO2.
- Analytical purity: reagent grade chemical
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River: Harlan Industries, Inc.
- Age at study initiation: 8-11-week-old-
Housing: Animals were group housed, separated by treatment group.
- Diet (e.g. ad libitum): Animals were allowed food (Purina Laboratory Chow) ad libitum.
- Water (e.g. ad libitum): Ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: waterAnimals were dosed with 1 mL of test solution.
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: Solutions were prepared gravimetrically in distilled water at concentrations of 1000 mg/L, 500 mg/L, and 200 mg/L.
- Duration of treatment / exposure:
- 5 days.
- Frequency of treatment:
- Daily (approximately 24 hr apart).
- Post exposure period:
- Animals were sacrificed 1, 3 and 5 weeks after dosing.
Doses / concentrationsopen allclose all
- Dose / conc.:
- 10 mg/kg bw/day (actual dose received)
- Remarks:
- 0.2 mg/day
- Dose / conc.:
- 25 mg/kg bw/day (actual dose received)
- Remarks:
- 0.5 mg/day
- Dose / conc.:
- 50 mg/kg bw/day (actual dose received)
- Remarks:
- 1 mg/day
- No. of animals per sex per dose:
- Ten male mice were used for each treatment group (three dose levels of the test solution and controls).
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Positive controls received 200 mg ethyl methanesulfonate/kg bw intraperitoneally in five equal doses.A positive control was used for each kill time.- The intraperitoneal (IP) route was used for administration of the positive control chemicals since this route was found to be suitable for eliciting consistent and effective responses.
Examinations
- Tissues and cell types examined:
- Mouse sperm.
- Details of tissue and slide preparation:
- The caudae epididymides were removed and diced in saline. The suspension was gently pipetted five to six times in and out of a 5- or 10-mL pipette. The suspension was strained through a 80-µm silk mesh to remove tissue fragments, and 0.5 mL of the filtrate was stained in a centrifuge tube with 0.05 mL 1 % Eosin Y. Slides were prepared from this suspension by spreading a drop over the slide with three passes of the edge of another slide.One thousand sperm-heads (500 by each of two readres) were scored per animal, where possible, for sperm-head shape abnormalities using the categories of Wyrobek and Bruce (1975).
- Evaluation criteria:
- A positive response was based upon a significant increase at any dose level over the concurrent control using a significance levelof 0.05.
- Statistics:
- Either an analysis of variance procedure or Student's t-test (a = 0.01) was used to analyze the data for differences in the percent sperm-head abnormalities per animal between treated animals and concurrent controls. In the case where reader differences were to be examined, a two-way (dose and reader) analysis of variance was done. Outliers were excluded from the analysis, as recommended by Soares et al (1979), using Dixon's Test for Outliers (1969) on the set of scores for each reader at each dose level. An arcsin transformation of the data was used to stabilize the variance for the analysis of variance.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
No treatment-related effects were observed.
Applicant's summary and conclusion
- Conclusions:
- No treatment-related effects were observed.
- Executive summary:
Male B6C3F1 mice (10 per group) were administered total doses of 0.2, 0.5 or 1 mg sodium chlorite by gavage in five equal daily doses. Positive controls received 200 mg ethyl methanesulfonate/kg bw intraperitoneally in five equal doses. Animals were sacrificed 1, 3 and 5 weeks after dosing, and the caudae epididymides were removed and diced in saline. Tissue fragments were filtered out, and 1000 sperm heads were scored per animal for abnormalities.
No treatment-related effects were observed.
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