4,4'-azobis[4-cyanovaleric] acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-azobis[4-cyanovaleric] acid

EC Number:

220-135-0

EC Name:

4,4'-azobis[4-cyanovaleric] acid

Cas Number:

2638-94-0

Molecular formula:

C12H16N4O4

IUPAC Name:

4,4'-azobis[4-cyanovaleric] acid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analysis of the test media samples was conducted on fresh media at 0 hours and on
corresponding old media at 48 hours

Test solutions

Vehicle:

no

Details on test solutions:

The definitive test was conducted at the following
nominal test substance concentrations: 30.7, 61.4, 123, 245 and 491 mg/L. The
491 mg/L treatment was pH adjusted prior to it being used to prepare the other test
concentrations. A control treatment was also included in the test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Juvenile Daphnia magna (less than 24 hours old) were taken from healthy laboratory
cultures maintained in media of the same composition and quality as that used in the
toxicity tests. These cultures were setup originally from dormant eggs (ephippia)
supplied byMicroBioTests Inc., Belgium.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Hardness (mg /L as CaCO3) 280

Test temperature:

19.4 – 20.7°C

pH:

7.44 - 7.73

Dissolved oxygen:

91 - 101%

Salinity:

Alkalinity (mg /L as CaCO3) 127

Conductivity:

639 - 986 (μS/cm)

Nominal and measured concentrations:

The measured test substance concentrations were between 66% – 122 % of nominal
test substance concentrations at 0 hours and 101 % - 120 % of nominal concentrations
at 48 hours.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 592 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 592 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes