1-amino-4-(ethylamino)-9,10-dihydro-9,10-dioxoanthracene-2-carbonitrile

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Specific details on test material used for the study:

Batch 135214 of Disperse Blue 359 was a dark blue powder with a purity of 99%.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 17 mg/L

Based on:

ThCO2

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

acetic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

> 0 - <= 3

Sampling time:

28 d

Remarks on result:

other: Disperse blue 359 not readily biodegradable based under conditions of the modified Sturm test presently performed.

Details on results:

Disperse blue 359 not readily biodegradable based under conditions of the modified Sturm test presently performed.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Disperse blue 359 not readily biodegradable based under conditions of the modified Sturm test presently performed.

Executive summary:

Since Disperse Blue 359 was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to the 2 -litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Test duration was 28 days (last CO2 -measurement on day 29). The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of Disperse Blue 359. In the toxicity control, Disperse Blue 359 was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid. Disperse Blue 359 was designated as not readily biodegradable.

Description of key information

Since Disperse Blue 359 was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to the 2 -litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test to ensure optimal contact between the test item and test organisms. Test duration was 28 days (last CO2 -measurement on day 29). The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of Disperse Blue 359. In the toxicity control, Disperse Blue 359 was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid. Disperse blue 359 not readily biodegradable based under conditions of the modified Sturm test presently performed.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed