1-amino-4-(ethylamino)-9,10-dihydro-9,10-dioxoanthracene-2-carbonitrile

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

>10% daphnids were immobilized/trapped at the surface of the control solution in the combined limit/range-finding test. In simultaneously performed tests no effects were observed in the control treatment, accept the results and use as a range-finder.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Readings taken every 24 hours for 48 hours

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (Crustacea, Cladocera) (Straus 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions. Tests ran with new born daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel.

Test type:

static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

48 h

Hardness:

180 mg/L expressed as CaCO3

Test temperature:

18-22 °C

pH:

7.7 ± 0.3

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 2.6 µg/L

Nominal / measured:

not specified

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Remarks on result:

not determinable

Remarks:

The 48h-EC50 was beyond range tested, i.e. exceeded the maximum solubility of Disperse Blue 359 in test medium (> 2.6 µg/L)

Details on results:

The 48h-EC50 was beyond range tested, i.e. exceeded the maximum solubility of Disperse Blue 359 in test medium (> 2.6 µg/L)

Validity criteria fulfilled:

yes

Conclusions:

A limit test was performed based on the results of a combined limit/range-finding test. Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an intreated control and to a saturated solution prepared at a loading rate of 100 mg/L. The total exposure period was 48 hours and samples or analytical confirmation of exposure concentrations were taken at the start and end of the test.
Samples taken from the limit concentration were analysed. The actual concentrations were below the LOD of the analytical method (LOD=5.1 µg/L) from the beginning of the exposure. Therefore, the effect parameters were expressed in terms of solubility in test medium, i.e. 2/6 µg/L (i.e. half of the LOD).
The study met the acceptability criteria prescribed by the study plan and was considered valid. The 48h-EC50 was beyond range tested, i.e. exceeded the maximum solubility of Disperse Blue 359 in test medium (> 2.6 µg/L).

Executive summary:

 

A limit test was performed based on the results of a combined limit/range-finding test. Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an intreated control and to a saturated solution prepared at a loading rate of 100 mg/L. The total exposure period was 48 hours and samples or analytical confirmation of exposure concentrations were taken at the start and end of the test.

Samples taken from the limit concentration were analysed. The actual concentrations were below the LOD of the analytical method (LOD=5.1µg/L) from the beginning of the exposure. Therefore, the effect parameters were expressed in terms of solubility in test medium, i.e. 2/6µg/L (i.e. half of the LOD).

The study met the acceptability criteria prescribed by the study plan and was considered valid. The 48h-EC50 was beyond range tested, i.e. exceeded the maximum solubility of Disperse Blue 359 in test medium (> 2.6µg/L).

Description of key information

 

A limit test was performed based on the results of a combined limit/range-finding test. Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an intreated control and to a saturated solution prepared at a loading rate of 100 mg/L. The total exposure period was 48 hours and samples or analytical confirmation of exposure concentrations were taken at the start and end of the test.

Samples taken from the limit concentration were analysed. The actual concentrations were below the LOD of the analytical method (LOD=5.1µg/L) from the beginning of the exposure. Therefore, the effect parameters were expressed in terms of solubility in test medium, i.e. 2/6µg/L (i.e. half of the LOD).

The study met the acceptability criteria prescribed by the study plan and was considered valid. The 48h-EC50 was beyond range tested, i.e. exceeded the maximum solubility of Disperse Blue 359 in test medium (> 2.6µg/L).

Additional information

 

A limit test was performed based on the results of a combined limit/range-finding test. Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an intreated control and to a saturated solution prepared at a loading rate of 100 mg/L. The total exposure period was 48 hours and samples or analytical confirmation of exposure concentrations were taken at the start and end of the test.

Samples taken from the limit concentration were analysed. The actual concentrations were below the LOD of the analytical method (LOD=5.1µg/L) from the beginning of the exposure. Therefore, the effect parameters were expressed in terms of solubility in test medium, i.e. 2/6µg/L (i.e. half of the LOD).

The study met the acceptability criteria prescribed by the study plan and was considered valid. The 48h-EC50 was beyond range tested, i.e. exceeded the maximum solubility of Disperse Blue 359 in test medium (> 2.6µg/L).