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EC number: 282-162-4 | CAS number: 84100-84-5
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1988.08.17 - 1988.09.14
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Type: mixed population of aquatic microorganisms (predominantly domestic sewage)
Origin: secondary effluent of a domestic sewage treatment plant (Wupper area water authority, STP Odenthal)
Pre-treatment of the inoculum
- separation of coarse particles by filtration
- aeration of filtrate - Duration of test (contact time):
- 28 d
- Initial conc.:
- 242.4 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Exposure conditions
- Test vessels: Closed bottles (Volume: 250 mL)
- Test concentration of the sludge: 5 mL/L
- Incubation time: 5, 15 and 28 days
- Incubation temperature: 20 +- 1 °C
Procedure
- mineral medium containing the test substance and inoculum was incubated under aerobic conditions in the dark
- endogenous activity of the inoculum was checked by running parallel blanks with inoculum but without test item
- reference compound (Aniline) was run in parallel to check the operation of the procedures
- Degradation was followed by the determination of oxygen uptake
- measurements were taken at frequent intervals to allow the identification of the beginning and end of biodegradation
- study period was 28 days
Pre-treatment of the test item
- Stock solution: 0.01 g of the test item was weighed out and added to 1 litre deionised water
- initial concentration of the test item was 242.4 mg/L
Pre-treatment of the reference compound
- Reference compound name: Aniline
- Stock solution: 1 g of the reference compound was weighed out and added to 1 litre deionised water. - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2
- Sampling time:
- 28 d
- Results with reference substance:
- The reference compound Aniline showed 66 % degradation after 15 days.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- After 28 d a biodegradation of 2 % was determined for 2,3,3-trimethyl-3H-indole-5-carboxylic acid at 20 °C. Thus the substance is not readily biodegradable.
- Executive summary:
A study was performed to assess the ready biodegradability of 2,3,3-trimethyl-3H-indole-5-carboxylic acid in accordance with the Council Regulation (EC) No 79/831, Method C.4-E “Closed Bottle Test“. This test method is in all essential parts identical with OECD Guideline 301 D.
A solution of 2,3,3-trimethyl-3H-indole-5-carboxylic acid in a mineral medium was inoculated and incubated for 28 d under aerobic conditions at 20 °C. During this period, degradation was followed by BOD determinations. A biodegradation of 2 % after 28 days was determined. Therefore 2,3,3-trimethyl-3H-indole-5-carboxylic acid is considered to be “Not Readily Biodegradable“.
The reference compound Aniline showed 66 % degradation after 15 days.
Reference
Description of key information
A study was performed to assess the ready biodegradability of 2,3,3-trimethyl-3H-indole-5-carboxylic acid in accordance with the Council Regulation (EC) No 79/831, Method C.4-E “Closed Bottle Test“. This test method is in all essential parts identical with OECD Guideline 301 D.
A solution of 2,3,3-trimethyl-3H-indole-5-carboxylic acid in a mineral medium was inoculated and incubated for 28 d under aerobic conditions at 20 °C. A biodegradation of 2 % after 28 days was determined. Therefore 2,3,3-trimethyl-3H-indole-5-carboxylic acid is considered to be “Not Readily Biodegradable“.
The reference compound Aniline showed 66 % degradation after 15 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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