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Short-term toxicity to fish

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Description of key information

LC50 (96 h) = 250 mg/L (BASF AG, 1995)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
250 mg/L

Additional information

The acute toxicity to fish was conducted according to OECD guideline 203 (BASF AG, 1995). 10 zebra fish (Brachyodanio rerio) per concentration were exposed to test substance concentrations of 0 (control), 21.5, 46.4, 100, 215 and 464 mg/L under static conditions. Mortality and sublethal effects were observed after 1, 4, 24, 48, 72 and 96 hours.
Mortality was observed at 464 mg/L. All animals were found dead after 24 hours. Tumbling was observed in the two highest testing groups. At 215 mg/L fish showed tumbling between 24 and 72 hours of exposure. In the highest testing group tumbling was observed after 4 hours.
Results were based on nominal and analytically determined values:

Results (nominal):
LC50 (96 hours) = 250 mg/L
NOEC = 100 mg/L

Results (analytical):
LC50 (96 hours) = 232 mg/L
NOEC = 89 mg/L

The analytically detected concentrations were initially in the range of 89.2% - 101.3% and after about 96 hours between 89.0%- 98.3% of the nominal concentrations.