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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Reported in 1950
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference
Reference Type:
publication
Title:
Dehydroacetic acid (DHA): 1. Acute and chronic toxicity
Author:
Spencer HC, Rowe VK & McCollister DD
Year:
1950
Bibliographic source:
J. Pharmacol. Exp. Therap., 99; 57-68

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Method was described as essentially like that of Draize et al., (1944) J. Pharmacol. Exp. Therap. 82; 377. Cited in Principles and Methods of Toxicology, 5th Edition; ed. A. Wallace-Hayes, 2008.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Dehydroacetic acid.

Test animals

Species:
rabbit
Strain:
not specified
Sex:
not specified

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: Greaseless base - "Neobase".
Details on dermal exposure:
After application of the dosage, the site was occluded by bandages for 24 hours after which time they
were removed and the site washed with soap and water.
Duration of exposure:
24 hours
Doses:
1000, 3000 or 5000 mg/kg bw
No. of animals per sex per dose:
2
Control animals:
no

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
approximate LD50
Effect level:
> 3 000 - < 5 000 mg/kg bw
Based on:
test mat.
Mortality:
One of the two rabbits treated at 5000 mg/kg bw died
Body weight:
Transitory weight loss

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
No rabbits died at 1000 or 3000 mg/kg bw. The LD50 was considered to be greater than 3000 mg/kg bw
Executive summary:

Rabbits were treated dermally for 24 hours with dihydroacetic acid at dosages between 1000 and 5000 mg/kg bw. There were no deaths at 1000 or 3000 mg/kg bw. The LD50 was considered to be greater than 3000 mg/kg bw.