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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Version / remarks:
May 31, 2008
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Version / remarks:
January 22, 2001
Guideline:
other: EC Technical Guidance Document on Risk Assessment Part III Chapter 4.3: Use of (Quantitative) Structure Activity Relationships ((Q)SARs) - Soil and Sediment Sorption.
Version / remarks:
2003
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil
Radiolabelling:
no
Details on study design: HPLC method:
EQUIPMENT
Instrument Acquity UPLC system
(Waters, Milford, MA, USA)
Detector Acquity UPLC TUV detector (Waters)
Column Acquity UPLC HSS Cyano,
100 mm 2.1 mm i.d., dp = 1.8 µm (Waters)
Column temperature 35°C 1°C
Injection volume 5 µL
Mobile phase 55/45 (v/v) methanol/0.01M buffer pH 5.5
Flow 0.4 mL/min
UV detection 210 nm

MOBILE PHASES
A stock solution of the reference substance at a concentration of approximately 1 g/L in methanol were used. The stock solution was diluted to obtain an end solution of 55/45 (v/v) methanol/0.01M buffer pH 5.5.
A 1000 mg/L stock solution of the test item was prepared in methanol. The stock solution was diluted to obtain an end solution of 55/45 (v/v) methanol/0.01M buffer pH 5.5. The final concentration of the test item solution was 50.0 mg/L.

REFERENCE SUBSTANCES
- Identity: Acetanilide

REPETITIONS
Reference substance and test item solutions were injected in duplicate. Blank solutions were analyzed by single injection.
Type:
Koc
Value:
< 18.2 dimensionless
pH:
5.5
Type:
log Koc
Value:
< 1.26 dimensionless
pH:
5.5
Details on results (HPLC method):
UPLC-UV chromatograms of the test item solution and the corresponding blank are shown in Figure 6 and Figure 7, respectively. In the chromatogram of the test item solution, one peak with a retention time of 0.862 minutes was observed. Under the same analytical conditions, the retention time of acetanilide (log Koc = 1.26) was 0.902 minutes. It was concluded that the log Koc of test item was < 1.26 (Koc < 1.82 101).
Validity criteria fulfilled:
yes
Conclusions:
It was concluded that the log Koc of test item was < 1.26 (Koc < 1.82 * 10^1).

Description of key information

Key value for chemical safety assessment

Koc at 20 °C:
18.2

Additional information