Sodium 1-amino-9,10-dihydro-9,10-dioxo-4-[(2,2,4-trimethylcyclohexyl)amino]anthracene-2-sulphonate

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

EC50 (24h) > 100 mg/l (nominal)

Toxicity to aquatic plants other than algae

ErC50 (7d): 11.29 mg/l (geom. mean), based on frond number

Additional information

Short-term toxicity to aquatic invertebrates

The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202, part I (1984). No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 100 mg/l; 10 % of mortality was observed in one of the two blank controls. The EC0 was identified to be 100 mg/l. Test medium appeared to be blue solution.

Toxicity to aquatic plants other than algae

The phytotoxicity of the test item on the freshwater aquatic plant Lemna gibba was assessed in accordance with method and procedures outlined into the OECD guideline 221.

In the main test, exponentially growing cultures of Lemna gibba were exposed to nominal concentrations 5.0, 10.0, 20.0, 40.0, 80.0 and 160 mg/l of the test item, over a period of 7 days in a static system under defined conditions.

The measured concentrations deviated more than 20 % from the nominal during the experiment in each concentration, therefore, biological results are based on the geometric mean of the measured test item concentrations.

The average specific growth rate, as well as yield, was statistically significantly different from the control group at the concentrations of 1.29, 2.54, 5.49, 10.97, 23.69 and 32.70 mg/l, based on on frond number, dry and fresh weight.  

NOEr/yC (7d): < 1.29 mg/l, based on frond number, dry and fresh weight

LOEr/yC (7d): 1.29 mg/l, based on frond number, dry and fresh weight

ErC50 (7d): 11.29 mg/l (95 % confidence limits: 9.211 – 14.089 mg/l), based on frond number

ErC50 (7d): 7.47 mg/l (95 % confidence limits: 6.058 – 9.212 mg/l), based on dry weight

ErC50 (7d): 8.61 mg/l (95 % confidence limits: 7.468 – 9.956 mg/l), based on fresh weight

EyC50 (7d): 4.68 mg/l (95 % confidence limits: 4.025 – 5.383 mg/l), based on frond number

EyC50 (7d): 3.06 mg/l (95 % confidence limits: 2.535 – 3.605 mg/l), based on dry weight

EyC50 (7d): 3.57 mg/l (95 % confidence limits: 3.228 – 3.924 mg/l), based on fresh weight

Justification for classification or non-classification

According to the CLP Regulation (EC n. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment when the following criteria are met:

A )Acute (short-term) aquatic hazard

Category Acute 1: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

B) Long-term aquatic hazard

(iii) Substances for which adequate chronic toxicity data are not available and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 1: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

Category Chronic 2: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants)> 1 to ≤10 mg/l

Category Chronic 3: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) > 10 to ≤ 100 mg/l

In the Lemna gibba study an ErC50 of 11.29 mg/l was found therefore the substance has been classified as toxic to aquatic compartment Category 3 according to the CLP Regulation (EC 1272/2008).