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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)

Data source

Reference Type:
study report

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
EC Number:
EC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Cas Number:
Molecular formula:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Test material form:
solid: crystalline

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
25 mg/L
Based on:
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution

Results and discussion

% Degradationopen allclose all
Key result
% degradation (CO2 evolution)
ca. 0 - ca. 5
Sampling time:
6 d
Key result
% degradation (CO2 evolution)
ca. 0 - ca. 17
Sampling time:
14 d
Key result
% degradation (CO2 evolution)
ca. 0 - ca. 17
Sampling time:
21 d
Key result
% degradation (CO2 evolution)
ca. 0 - ca. 16
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
not readily biodegradable
The test item must be regarded to be not readily biodegradable in the 10-d-window and after 28 days.
Executive summary:

The ready biodegradability was determined with a non adapted activaled sludge for the test item AMINOL (batch no.: R0075037) over a test period of 28 days in the Modified Sturm Test. The study was conducted from 2003-05-15 to 2003-06-13 according to OECD 301 B/C02 evolution test in the DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE. The test item was tested in a concentration of 25 mg/L in duplicates, corresponding to a carbon content (TOC) of 12.1 mgC/L in the test vessels. The biodegradation of the test item was followed by titrimetric analyses of the quantity of CO2 , which was produced by the respiration of bacteria. The degradation was finished on day 28 by acidification, the last titration was made on day 29, after the soluble CO2 was turned out over a period of 24 h. The CO2 production was calculated as the percentage of total CO2 that the test item could have theoretically produced based on carbon content. Biodegradation is therefore expressed as percentage ThCO2 and was calculated for each titration of CO2. ln order to check the activity of the test system sodium acetate was used as functional control. The percentage degradation of the functional control reached the pass Ievel of 60% after 14 days. The validity criterion of the guideline is fulfilled. ln the toxicity control containing both test and reference item a biodegradation rate of 40% occurred within 14 days and came to a maximum of 76 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control. The biodegradalion of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. The 10 % level (beginning of biodegradation) was reached only by one replicate after 8 days. The second replicate remained at a biodegradation of 0 %. ln the 10 -d-window the pass level of a biodegradation of more than 60 % was not reached. The biodegradation of the first replicate came to a maximum of 19% after 18 days. At this point the plateau was reached. The validity criteria according to the guideline are fulfilled.

The test item must be regarded to be not readily biodegradable in the 10-d-window and after 28 days.

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