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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
EC Number:
303-085-5
EC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Cas Number:
94158-14-2
Molecular formula:
C9H11NO3.ClH
IUPAC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Test material form:
solid: crystalline

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
18-22 °C +/- 1 °C

Results and discussion

Effect concentrations
Key result
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
2.65 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The Acute Immobilisation (EC50) of the test item AMINOL (batch number: R0075037), to Daphnia magna (STRAUS) was determined according to OECD Guideline 202 (1999, Draft)/EC Directive 92/69/EC Method C.2 from 2003-08-26 to 2003-08-28 at the DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE, D-31157 Sarstedt. The study was conducted under static conditions over a period of 48 hours. 20 test organisms were exposed to each test concentration and control. The recovery rates of the test item in the new and the old media were > 80 %, therefore the results are given based on the nominal test item concentrations. The nominal test item concentrations were as follows: 10.0 - 5.00 - 2.50 - 1.25 - 0.625 mg/L. The 24 and 48 h EC10-values and the 24 and 48 h EC50-values with confidence interval (Cl) were calculated by probit analysis (Table 1 ). The 24 and 48 h EC100-values were determined directly from the test results obtained. A reference test was carried out with potassium dichromate. The EC50-value of the reference item at 1.49 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable Iimits. The validity criteria of the test guideline were fulfilled.

Table 1: EC10-, EC50- (with Confidence lnterval), EC100- Values

(based on nominal test item concentrations)

 

Test duration [h]

Concentration [mg/L]

Confidence interval (p=95%) [mg/L]

EC10

24

2.65

---

EC10

48

2.15

---

EC50

24

6.01

4.79-7.56

EC50

48

2.67

2.48-2.87

EC100

24

>10.0

---

EC100

48

5.00

---

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