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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
ln contrast to the guideline, it was decided to use copper (II) sulphate pentahydrate instead of the prescribed 3,5-dichlorophenol. The usage of Dichlorophenol is unsuitable for routine Iab use
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
EC Number:
303-085-5
EC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride
Cas Number:
94158-14-2
Molecular formula:
C9H11NO3.ClH
IUPAC Name:
2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h

Results and discussion

Effect concentrations
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
77 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration

Applicant's summary and conclusion

Conclusions:
The EC50 -value for the test item AMINOL is 77 mg/L.
Executive summary:

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was performed for 3 h with the test item AMINOL A098 (batch-no. R0075037), on 2003-05-08 at the test facility DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE, D-31157 Sarstedt. Testsystem was activated sludge of the municipal treatment plant of D-31157 Sarstedt. The test method was static. Nominal concentrations of the test item were as follows: 10- 32- 100- 320- 1000- 3200 mg/L. The respiration rates of control, reference and test item were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. The concentration effect relationship is shown in Figure 1. The Inhibition ranged from 28 to 81% (Table 2). The EC20 , EC50 - and EC80 -values were calculated via probit analysis (Table 1 ). ln order to check the activity of the test system and the test conditions a reference test is carried out with copper-(11)-sulphate-pentahydrate as reference item and the reference toxicity is determined. The EC50 - value for the reference item was 105 mg/L.

Validity criteria of the lest guideline were fulfilled.

Table 1: EC-values with Confidence Interval of AMINOL A098

   EC values (mg/L)  Confidence interval P=95% (mg/l)
 EC20  < 10 (2.4)*  -
 EC50  77  48 -123
 EC80  2499  -

*Extrapolation The EC50 -value for the test item AMINOL is 77 mg/L.