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EC number: 222-824-1 | CAS number: 3623-51-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to IUCLID section 13 for a detailed justification of the category approach.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 37.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 18.8-75.6 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 11.35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 14.7-48.2 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 92.35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Please, see the section "any other information on results incl. tables" for a tabulated listing of the degree of immobilisation observed in the test animals at different concentrations of the test item and the two images attached below for graphical representations of the dose-response-relationship observed at 24 and 48 h, respectively.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In this acute toxicity study in Daphnia magna, the effective concentration 0% (EC0) and the effective concentration 100% (EC100) for immobilisation induced by an exposure to the test item for 24 h were determined as 25 and 100 mg/L, respectively. For an exposure period of 48 h, the respective values were 11.35 and 92.35 mg/L. Furthermore, effective concentration 50% (EC50) values of 37.7 and 26.6 mg/L were determined for the 24 h and the 48 h exposure, respectively.
- Executive summary:
The acute toxicity of the test item, was tested in an immobilisation assay in Daphnia magna. The assay was conducted according to EU method C.2. The test organisms were exposed the substance at nominal concentrations of 3.2, 6.3, 12.5, 25, 50, and 100 mg/L for up to 48 h. Test subjects were observed for alterations of the normal mobility behaviour and loss of locomotory activity. From the data obtained through these observations, the effective concentration 0% (EC0) and the effective concentration 100% (EC100) for immobilisation induced by an exposure to the test item for 24 h were determined as 25 and 100 mg/L, respectively. For an exposure period of 48 h, the respective values were 11.35 and 92.35 mg/L. Furthermore, effective concentration 50% (EC50) values of 37.7 and 26.6 mg/L were determined for the 24 h and the 48 h exposure, respectively.
Reference
Immobilisation of Daphnia magna after an exposure of 24 h
Immobilisation of Daphnia magna (initial population: 20) |
0 mg/L |
3.2 mg/L |
6.3 mg/L |
12.5 mg/L |
25 mg/L |
50 mg/L |
100 mg/L |
Absolute |
0 |
0 |
0 |
0 |
0 |
4 |
16 |
Cumulative |
0 |
0 |
0 |
0 |
0 |
4 |
16 |
Cumulative (%) |
0 |
0 |
0 |
0 |
0 |
20 |
80 |
Immobilisation of Daphnia magna after an exposure of 48 h
Immobilisation ofDaphnia magna (initial population: 20) |
0 mg/L |
3.2 mg/L |
6.3 mg/L |
12.5 mg/L |
25 mg/L |
50 mg/L |
100 mg/L |
Absolute |
0 |
0 |
0 |
0 |
4 |
2 |
20 |
Cumulative |
0 |
0 |
0 |
0 |
4 |
18 |
20 |
Cumulative (%) |
0 |
0 |
0 |
0 |
20 |
90 |
100 |
Description of key information
Daphnia magna EC50 (48 h) = 26.6 mg/L (measured); read-across
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 26.6 mg/L
Additional information
Since no data for the target substance are available, the aquatic
toxicity is assessed on the basis of data from one structurally similar
menthol substance (stereo isomer).
The acute toxicity of L-menthol (CAS 2216-51-5) was tested in a static
immobilisation test with the water flea Daphnia magna as test organism.
The study was conducted according to EU method C.2 (GLP). The test
organisms were exposed to nominal concentrations of 3.2, 6.3, 12.5, 25,
50, and 100 mg/L for up to 48 h. Test organisms were observed for
alterations of the normal mobility behaviour and loss of locomotory
activity. From the data obtained during these observations, the
effective concentration 0% (EC0) and the effective concentration 100%
(EC100) for immobilisation induced by an exposure to the test item for
48 h were determined as 11.35 and 92.35 mg/L, respectively. The
effective concentration 50% (EC50) value was calculated to be 26.6 mg/L.
Based on the result from one structurally related source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) it can be concluded that testing the target substance would result in comparable results.
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