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EC number: 222-824-1 | CAS number: 3623-51-6
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- Density
- Particle size distribution (Granulometry)
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- Additional physico-chemical information
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Mar 14, 2000 - Mar 17, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Origin: Institute of Plant Physiology, University of Goettingen
- Algal inoculum 10000 cells/ml initial cell density
- Illumination: 120 µE/m2s
- Cell density measured with a microcelcounter after 24, 48 and 72 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- pH:
- 8.0 - 10.3
- Nominal and measured concentrations:
- Nominal concentration: 1.25, 2.5, 5, 10 and 20 mg/l
- Details on test conditions:
- - Erlenmeyer flasks (300 ml) with stoppers
- 100 ml of test medium
- Light chamber with shaker - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 15.504 - 16.537 mg/l
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Since the original study on short-term toxicity to algae from 2000 only gives a range (> 9.8 < 18.2 mg/L) but not a distinct value for the ErC50 this value was determined by Probit analysis as ErC50 = 16.2 mg/L and quoted in the OECD SIDS report, 2003 (agreed by the ICCA initiative). The calculated ErC50 value is used as result of the underlying study on short-term toxicity to algae.
- Validity criteria fulfilled:
- yes
- Conclusions:
- During 72 hours exposure an ErC50 of 16.2 mg/L and a NOEC of 4.6 mg/l was determined.
- Executive summary:
With a growth inhibition study according to EU Method C.3 on algae (Desmodesmus subspicatus former name: Scenedesmus subspicatus) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 a 72-hour EC50 of 16.2 mg/l (based on the measured arithmetic mean) was determined for DL-Menthol (CAS no. 1490-04-6). The EC50 value is quoted in the OECD SIDS report, 2003 (agreed by the ICCA initiative).The NOEC resulting from the Dunnett test was 4.6 mg/l.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Dec 10, 2002 - Dec 13, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- (2002-12-19)
- Analytical monitoring:
- yes
- Details on sampling:
- Schedule of sampling:
Stock solution at 0 hours
Control at 0 and 72 hours
Test concentration at 0 and 72 hours - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Before the beginning of the study, the test substance was pulverized. A stock solution was prepared to give the desired series of test concentrations. To achieve this 124.9 mg of the test substance were added to 1 litre of dilution water and treated for 1 h in an ultrasonic bath and afterwards stirred for 24 h on a magnetic stirrer. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: Non-axenic strain
- Source (laboratory, culture collection): "The collection of Algal Cultures" of the Institute of Plant Physiology at the University of Göttingen, Germany.
- Method of cultivation: Exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions (23 +/- 2°C) at a light intensity in the range 60-120 μE/m2/s (measured in the range 400 to 700 nm using a spherical quantum flux meter). The nutrient medium (according to BRINGMANN & KÜHN (1977)) is renewed once a week. Cell density measurements are made using a microcell counter.
ACCLIMATION
- Acclimation period: Pre-cultures are set up three days before the start of a test.
- Culturing media and conditions (same as test or not): Same as test culture method (see above) - Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21°C to 25°C +/- 2°C
- pH:
- At 0 hour: pH=8.2 to 8.5
At 72 hours: pH=8.6 to 10.4 - Nominal and measured concentrations:
- Nominal concentrations: 5.0, 10, 20, and 40 mg/l administered from stock solutions.
Measured concentrations (at 0 hour - at 72 hours): 4.6-4.5, 10-9.3, 19.9-17.6 and 41-36.7 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 ml Erlenmeyer flasks with stoppers
- Initial cells density: 10^4 cells/ml
- Control end cells density: 318889 cell/ml
- No. of vessels per concentration (replicates): 3 replicates per concentration
- No. of vessels per control (replicates): 6 replicates per concentration
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Photoperiod: continuous uniform illumination
- Light intensity and quality: light between 400-700 nm, light intensity in the range 60-120 μE/m2/s (equivalent to 4000 to 8000 lx)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microcell counter or alternatively by means of a microscopic counting chamber
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x2
- Test concentrations: see above - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: reduction of growth
- Remarks on result:
- other: 95% confidence limits : 16-24 mg/l
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limits : 21.4-21.5 mg/l
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.65 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: reduction of growth
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.65 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 18.75 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: reduction of growth
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 18.75 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the test using the freshwater algae Desmodesmus subspicatus an ErC50 (based on growth rate) of 21.4 mg/l was determined, whereas the No effect concentration (NOEC) was determined to be 9.65 mg/l. The substance needs to be considered harmful to algae.
Referenceopen allclose all
Results analysis by start of test and after 72 hours
Nominal concentration [mg/l] | 0 hours [mg/l] | 72 hours [mg/l] |
control | < 2 | 3 |
5 *w.algae | 5 | 3 |
5 | 4 | 3 |
10 | 8 | 7 |
20 | 15 | 13 |
40 | 29 | 26 |
40 *w.algae | 27 | 27 |
*w.algae = without algal inoculum
All values refer to TOC determinations. According to the relevant product infomations 1 mg/l TOC equals to 1.3 mg/l of the test substance (empirical formula: C10H20O; molecular weight: 156,3g/mol).
Analysis of the test concentrations 5 mg/l, 10 mg/l, 20 mg/l, 40 mg/l plus a control; additional analysis of the highest and the lowest analytically determined test concentration without algal inoculum,at test start and after 72 hours of exposure. The test concentrations 1.25 mg/l and 2.5 mg/l were not analytically determined as the measured concentrations to be expected were below the detection limit of the TOC determination method (2 mg/l).
Before measuring TOC, the samples after 72 hours of exposure were filtered.
Description of key information
Desmodesmus subspicatus ErC50 (72 h) = 16.2 mg/L (measured, based
on growth); read-across
Desmodesmus subspicatus NOErC (72 h) = 4.6 mg/L (measured, based on
growth); read-across
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 16.2 mg/L
- EC10 or NOEC for freshwater algae:
- 4.6 mg/L
Additional information
Since no data for the target substance are available, the aquatic
toxicity is assessed on the basis of data from two structurally similar
menthol substances (stereo isomers).
Both studies with the source substances examined the impact on growth of
the unicellular green algae Desmodesmus subspicatus according to
standard guidelines and GLP. In the key study with D/L-menthol (CAS
1490-04-6) a growth inhibition of 50% (ErC50, 72 h) was observed in the
range of > 9.8 < 18.2 mg/L test item concentration (measured arithmetic
mean). By Probit analysis a defined value for the ErC50 (72 h) of 16.2
mg/L (measured arithmetic mean) was determined. The corresponding NOErC
(72 h, based on growth rate) is reported with 4.6 mg/L (measured
arithmetic mean).
This result is confirmed by the supporting study with L-Menthol (CAS
2216-51-5), where a slightly higher ErC50 (72 h) of 21.4 mg/L (measured
arithmetic mean) was determined. The corresponding NOErC (72 h, based on
growth rate) is reported to be 9.65 mg/L (measured arithmetic mean).
Based on the results from two structurally related source substances (in
accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) it can be
concluded that testing the target substance would result in comparable
results.
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