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EC number: 222-824-1 | CAS number: 3623-51-6
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- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
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- Flash point
- Auto flammability
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- Stability: thermal, sunlight, metals
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- Dissociation constant
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- Endpoint summary
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jun 01, 1991 - Jun 03, 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Remarks:
- The study was performed acording to a method similar to the one described in OECD guideline No. 209 and followed the principles of GLP. However, relatively few details are provided in the study documentation.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- no data
- Vehicle:
- not specified
- Details on test solutions:
- no details provided
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Laboratory culture: The activated sludge was obtained on 29.05.1992 from a laboratory water treatment plant (OECD).
Pretreatment: None
Initial biomass concentration: 6 g/L dry weight - Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- no data
- Hardness:
- no data
- Test temperature:
- no data
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- nominal concentrations: 100, 180, 320, 560, and 1000 mg/L
- Details on test conditions:
- Autoxidation was determined as 1000 mg/L.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 237 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Please, see section "Any other information on results incl. tables" for the values for the inhibition of activated sludge respiration observed with different concentrations of the test item and the attached image below for a graphical presentation of the results in an inhibition vs. concentration-diagram.
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity of the test system was checked by a graphical evaluation of the results obtained with the reference substance.
- Conclusions:
- The effective concentration 50% (EC50) for inhibition of activated sludge respiration was determined as 237 mg/L. In view of this low bacteriotoxic activity, it was concluded that release of the test compound into the environment would most likely not adversely affect the natural self purification capacity of surface water bodies.
- Executive summary:
The toxicity of the test item, Menthol H&R Komp., to microorganisms was tested in an activated sludge respiration assay. This assay was performed according to the ISO guideline 8192 -1986 (E), which largely resembles the OECD guideline No. 209. In this assay, The effective concentration 50% (EC50) for inhibition of activated sludge respiration by the test item was determined as 237 mg/L.
Since this value indicates a rather low bacteriotoxic activity, it was concluded that release of the test compound into the environment would most likely not adversely affect the natural self purification capacity of surface water bodies.
The validity of the test system was checked by testing a confirmed bacteriotoxic compound, 3,5-dichlorophenol, as reference substance.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Dec 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Initial biomass concentration: 6.0 g/L (dry weight)
- laboratory scale sewage treatment plant treating synthetic sewage (OECD 209) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.8 °C
- pH:
- 8.0 - 8.3
- Dissolved oxygen:
- O2 - Start: 5.2 - 8.4 mg
O2 - End: 3.0 - 8.2 mg - Nominal and measured concentrations:
- nominal concentrations: (preliminary Test) 100, 1000 and 10000 mg/L
nominal concentrations: (primary test) 100, 180, 320, 560 and 1000 mg/L - Details on test conditions:
- Type: aquatic
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 306 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The concentrations of the test item and reference compound were not confirmed by analytical methods, but instead based on nominal concentrations
- Results with reference substance (positive control):
- EC50 of the reference compound 3.5-Dichlorophenol is < 20 mglL
- Validity criteria fulfilled:
- yes
- Remarks:
- on the basic of graphic evaluation; The EC50 for 3,5-DCP was 20 mg/L and between the prescribed limits of 5 and 30 mg/L.
- Conclusions:
- For DL-Menthol (CAS no. 1490-04-6) an EC50 (3 h) of 306 mg/L was observed.
- Executive summary:
A study with activated sludge with a duration fo 3 hours was performed according to the OECD Guideline 209. For DL-Menthol (CAS no. 1490-04-6) an EC50 of 306 mg/L was observed.
Referenceopen allclose all
Table 1: Results of the activated sludged respiration inhibition assay
Test concentration (mg/L) |
Respiration rate (mg/L x h) |
Inhibition (%) |
100 |
28.3 |
18.7 |
180 |
20.3 |
41.7 |
320 |
11.3 |
67.5 |
560 |
7.0 |
79.9 |
1000 |
6.0 |
82.8 |
Results:
- preliminary Test:
Test conc. [mg/l] | Respirations rate [mg/l*h] | Inhibition [%] | Referenz substance [mg/l] | Respirations rate [mg/l*h] | Inhibition [%] |
100 | 22 | 0 | 1.0 | 22 | 0 |
1000 | 3 | 85 | 20 | 5 | 75 |
10000 | 0 | 100 |
Results:
- primary Test:
Test conc. [mg/l] | Respirations rate [mg/l*h] | Inhibition [%] | Referenz substance [mg/l] | Respirations rate [mg/l*h] | Inhibition [%] |
100 | 19 | 7 | 1.0 | 22 | 0 |
180 | 17 | 19 | 20 | 5 | 75 |
320 | 7 | 67 | |||
560 | 4 | 80 | |||
1000 | 2 | 88 |
Description of key information
Activated sludge respiration inhibition EC50 (3 h) = 237 mg/L (nominal); EC10 (3 h) = 51 mg/L (nominal); read-across
Key value for chemical safety assessment
- EC50 for microorganisms:
- 237 mg/L
- EC10 or NOEC for microorganisms:
- 51 mg/L
Additional information
Since no data for the target substance are available, the toxicity
to activated sludge microorganisms is assessed on the basis of data from
two structurally similar menthols (stereo isomers).
The toxicity to microorganisms was investigated for L-menthol (CAS
2216-51-5) and an unspecified menthol isomer mixture (D/L-menthol, CAS
1490-04-6). The studies with both source substances investigated the
respiration inhibition of activated sludge microorganism under standard
test conditions in accordance to GLP.
In the key study with L-menthol (CAS 2216-51-5), which was conducted
according to ISO 8192 (similar to OECD 209), an EC50 of 237 mg/L
(nominal) and an EC10 of 51 mg/L (nominal) were determined after 3 h
based on inhibition of respiration. The test system was validated using
3,5-dichlorophenol as reference substance.
This result was confirmed in the supporting study performed with the
source substance D/L-menthol (CAS 1490-04-6). This study was conducted
according to OECD 209 (GLP). An EC50 of 306 mg/L (nominal) was observed
after 3 h of exposure.
Based on the results from two structurally related source substances (in
accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) it can be
concluded that testing the target substance would result in comparable
results.
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