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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from authoritative database.

Data source

Reference
Reference Type:
other: Authoritative database
Title:
Ocular irritation study of test substance
Author:
U. S. National Library of Medicine
Year:
2018
Bibliographic source:
HSDB (Hazardous Substances Data Bank); US national Library of Medicine reviewed by SRC, 2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 870.2400 (Acute Eye Irritation)
Principles of method if other than guideline:
The potential of test chemical to induce eye irritation was assessed in 9 rabbits according to methods similar to OPPTS 870.2400 and OECD 405 guidelines.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium phosphinate
EC Number:
231-669-9
EC Name:
Sodium phosphinate
Cas Number:
7681-53-0
Molecular formula:
H3O2P.Na
IUPAC Name:
Sodium hypophosphite
Test material form:
solid
Details on test material:
- Name of test material: sodium phosphinate
- IUPAC name: Sodium hypophosphite
- Molecular formula: NaO2P
- Molecular weight: 87.9778 g/mole
- Smiles notation: [Na+].[O-]P=O
- InChl: 1S/Na.H3O2P/c;1-3-2/h;3H2,(H,1,2)/q+1;/p-1
- Substance type: Inorganic
- Physical state: Solid

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
not specified

Test system

Vehicle:
not specified
Controls:
other: The other untreated eye served as control.
Amount / concentration applied:
100 mg
Duration of treatment / exposure:
72 hours
Observation period (in vivo):
24, 48 and 72 hours after the administration and then daily until reversibility of the ocular reactions.
Number of animals or in vitro replicates:
3 (Male/female)
Details on study design:
TEST SITE
- Area of exposure: one eye
REMOVAL OF TEST SUBSTANCE
- Washing (if done): For 6 rabbits, the eyes were not rinsed after administration of the test item while a washing procedure was applied for 3 other animals.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
chemosis score
Remarks:
For rinsed eye
Basis:
mean
Time point:
72 h
Score:
0.33
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritation parameter:
conjunctivae score
Remarks:
For rinsed eye
Basis:
mean
Time point:
72 h
Score:
0.89
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritation parameter:
iris score
Remarks:
For rinsed eye
Basis:
mean
Time point:
72 h
Score:
0
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritation parameter:
cornea opacity score
Remarks:
For rinsed eye
Basis:
mean
Time point:
72 h
Score:
0
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
For rinsed eye : 0.33 for chemosis, 0.89 for redness of the conjunctiva, 0 for iris lesions and 0 for corneal opacity. Redness and chemosis were fully reversible within 10 and 3 days respectively.
Other effects:
not specified

Any other information on results incl. tables

Washing procedure did not change the ocular reactions.

Applicant's summary and conclusion

Interpretation of results:
other: Not irritating
Conclusions:
Based on the observation and under the test experimental condition, the test chemical was considered as non-irritant to the eye of treated rabbits.
Executive summary:

An acute ocular irritation study was conducted for the given test chemical to determine the potential to induce eye irritation in 9 rabbits according to methods similar to OPPTS 870.2400 and OECD 405 guidelines. During the study, a single dose of 100 mg of the test chemical was instilled into one eye, the other eye was not treated and served as control. For 6 rabbits, the eyes were not rinsed after administration of the test chemical while a washing procedure was applied for 3 other animals. Ocular reactions were observed 24, 48 and 72 hours after the administration and then daily until reversibility of the ocular reactions. The values of the scores for chemosis, redness of the conjunctiva, iris lesions and corneal opacity were noted for each animal. Mean scores calculated over 24, 48 and 72 hours for the 6 animals without rinsing were 0.33 for chemosis, 0.89 for redness of the conjunctiva, 0 for iris lesions and 0 for corneal opacity. Redness and chemosis were fully reversible within 10 and 3 days respectively. Washing procedure did not change the ocular reactions. Thus, under these experimental conditions, test chemical was considered as non-irritant when administered by ocular route to rabbits.