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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from research article.

Data source

Reference
Reference Type:
review article or handbook
Title:
skin sensitization study of test chemical
Author:
The MAK Collection for Occupational Health and Safety
Year:
2017
Bibliographic source:
The MAK Collection for Occupational Health and Safety, 2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
A mouse local lymph node assay (LLNA) was conducted for test chemical to determine its sensitizing effects.
GLP compliance:
not specified
Type of study:
mouse local lymph node assay (LLNA)

Test material

Constituent 1
Chemical structure
Reference substance name:
Barium chloride (BaCl2), dihydrate
EC Number:
600-412-6
Cas Number:
10326-27-9
Molecular formula:
BaCl2
IUPAC Name:
Barium chloride (BaCl2), dihydrate
Test material form:
solid
Details on test material:
- Name of test material: Barium chloride (BaCl2), dihydrate
- Molecular formula: BaCl2
- Molecular weight: 244.2656 g/mole
- Smiles notation: [Ba](Cl)Cl.O.O
- InChl: 1S/Ba.2ClH.2H2O/h;2*1H;2*1H2/
q+2;;;;/p-2
- Substance type: Inorganic
- Physical state: Solid

In vivo test system

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
not specified

Study design: in vivo (LLNA)

Vehicle:
not specified
Concentration:
5 %, 10 %, and 25 % of the test substance (w/w)
No. of animals per dose:
No data available
Details on study design:
No data available
Positive control substance(s):
not specified
Statistics:
No data available

Results and discussion

In vivo (LLNA)

Resultsopen allclose all
Parameter:
SI
Value:
1.3
Remarks on result:
other: at 5%
Parameter:
SI
Value:
1.5
Remarks on result:
other: at 10%
Parameter:
SI
Value:
1.2
Remarks on result:
other: at 25%
Cellular proliferation data / Observations:
the chemical did not have sensitizing effects up to concentrations of 25%

Applicant's summary and conclusion

Interpretation of results:
other: not sensitizing
Conclusions:
The test chemical did not have sensitizing effects up to concentrations of 25%. Hence the chemical was considered as not sensitizing.
Executive summary:

A mouse local lymphnode assay (LLNA) was conducted for test chemical to determine its sensitizing effects. In this study, the application of 5%, 10% and 25% test chemical resulted in stimulation indices of 1.3, 1.5 and 1.2, respectively. Thus, a tripling of the lymphocyte stimulation was not reached with any of the test concentrations. Since, the chemical did not have sensitizing effects up to concentrations of 25%, it was considered as not sensitizing.