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EC number: 271-928-3 | CAS number: 68630-95-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72 hours
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- OECD Guidelines for Testing of Chemicals. Test Guideline 201, Freshwater Alga and Cyanobacteria, Growth Inhibition Test. Adopted 23 March 2006
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test substance name Crodateric CYAP
Scymaris reference number 1014TS047
Physical state and appearance Dark solid
Supplier Croda Europe Ltd.
Lot/batch number PS-196-555 - Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- The culture medium used for the test, and for the maintenance of cultures of the alga used as inoculum for the test was AAP-medium
- Details on test solutions:
- This study was run with a culture medium control and nominal exposure concentrations of 0.19, 0.609, 1.95, 6.25 and 20 mg/L.
Control and mean measured concentrations of 0.18, 0.57, 1.8, 5.8 and 18 mg/L
A primary stock concentrate of Crodateric CYAP with a nominal concentration of 20 mg/L was prepared by weighing a nominal 0.02 g of test substance (actual weight: 0.01992 g) and making up to 1000 mL volume with the culture medium AAP in a volumetric flask. The stock was stirred for 40 minutes. The resultant stock was observed to be a clear and colourless solution and was used to prepare the test solutions. This was achieved by adding the relevant volumes of the primary stock to AAP media and making up to 1000 mL volume in a volumetric flask. The 20 mg/L stock was used directly for the 20 mg/L blank and test vessels. The control consisted of culture medium only. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test species was the unicellular green alga Pseudokirchneriella subcapitata strain CCAP 278/4 from laboratory cultures maintained under axenic conditions. A 3-day old culture of the alga in the exponential growth phase was used as inoculum for the test. The culture was grown in the medium, and under the environmental conditions, described for the test.
- Test type:
- static
- Water media type:
- other: Cultre medium
- Total exposure duration:
- 72 h
- Test temperature:
- 22 +/- 2C
- pH:
- At the start of the test the pH ranged from 7.51 and 8.02 and at the end of the test the range was from 7.67 to 7.76
- Details on test conditions:
- The test vessels were glass conical flasks of 250 mL nominal capacity closed with foam bungs. Each flask contained 100 mL of test solution. The cultures were incubated at 22 +/- 2°C (the nominal test temperature), under continuous "cool-white" illumination of approximately 6000 lux, with nominal orbital shaking at 160 rpm.
Six replicates of the culture medium control and triplicates of each concentration of the test substance were employed. The position of each test replicate vessel in the incubator was randomised daily. One blank vessel (without algal inoculum) was incubated concurrently for each control and test concentration sampling occasion.
The algal cell densities of the inoculum and test cultures were determined by electronic particle counting, using a Coulter counter and counting between a lower and upper threshold equivalent spherical diameter of approximately 2.3 and 5.0 µm respectively. Each replicate test vessel was inoculated with 0.514 mL of the inoculum culture to give a nominal cell density of 0.5 × 104 cells/mL. One 100 mL volume of Coulter electrolyte, inoculated in the same manner, had a cell density of 0.539 × 104 cells/mL. This value was used for growth calculations.
After 24, 48, and 72 hours, samples were removed from each test and blank vessel. The appropriate blank particle count was subtracted from that of the test culture to obtain the cell density.
At the end of the test microscopic observations were made on samples taken from a single replicate of the control and each test substance concentration. - Reference substance (positive control):
- yes
- Remarks:
- culture medium
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: EyC50
- Effect conc.:
- 13.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Details on results:
- The microscopic observations, made at the end of the test, showed that compared to the control the algal cells sampled from the nominal 0.19, 0.609, 1.95, 6.25 mg/L test concentrations appeared normal and the cells sampled from the nominal 20 mg/L test concentration appeared deformed
- Validity criteria fulfilled:
- yes
- Conclusions:
- The microscopic observations, made at the end of the test, showed that compared to the control the algal cells sampled from the nominal 0.19, 0.609, 1.95, 6.25 mg/L test concentrations appeared normal and the cells sampled from the nominal 20 mg/L test concentration appeared deformed.
The NOEC level for both growth rate and yield for this substance was determined to be 6.25 mg/l and the LOEC level for both growth rate and yield was determined to be 20 mg/l
Reference
Growth rates - The growth rate (0 to 72 hours) was calculated for each replicate culture (Table 3), according to the formula:
where N1 = Cell density at start of test
N2 = Celldensity at end of test
t = Time interval (days)
The control was compared to the treatments by one-way analysis of variance and Bonferroni adjusted t test to identify significant differences (p<0.05). ECXwas calculated using the linear interpolation (ICPIN) method.
The mean growth rates, together with the rates expressed as percentages of the control.
The results obtained from these growth rate analyses, based on mean measured test concentrations, were as follows:
|
Crodateric CYAP (mg/L) |
95% confidence limits (mg/L) |
NOEC |
6.25 |
- |
LOEC |
20 |
- |
ErC50 |
>20 |
N/A |
ErC20 |
13.7 |
11.7-15.3 |
ErC10 |
8.90 |
6.58-10.7 |
Yield - This response is defined as the biomass at the end of the test minus the starting biomass. For the purposes of calculation, the cell particle density count (cell particles per unit volume) is an acceptable surrogate for biomass. The control was compared to the treatments by one-way analysis of variance and Bonferroni adjusted t test to identify significant differences (p<0.05). ECXwas calculated using the linear interpolation (ICPIN) method.
The EC50, EC20and EC10values with their associated confidence intervals were subsequently calculated using the Linear Interpolation method. The yield mean values are shown inTable 6, together with the yields expressed as percentages of the control.
The results obtained from these statistical analysis, based on mean measured test concentrations, were as follows:
|
Crodateric CYAP (mg/L) |
95% confidence limits (mg/L) |
NOEC |
6.25 |
- |
LOEC |
20 |
- |
EyC50 |
13.7 |
11.1-15.5 |
EyC20 |
6.74 |
N/A |
EyC10 |
1.93 |
N/A |
Description of key information
The microscopic observations, made at the end of the test, showed that compared to the control the algal cells sampled from the nominal 0.19, 0.609, 1.95, 6.25 mg/L test concentrations appeared normal and the cells sampled from the nominal 20 mg/L test concentration appeared deformed.
The NOEC level for both growth rate and yield for this substance was determined to be 6.25 mg/l and the LOEC level for both growth rate and yield was determined to be 20 mg/l.
ErC50 Growth rate = >20 mg/l. EyC50 Yield = 13.7 mg/l
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 20 mg/L
- EC10 or NOEC for freshwater algae:
- 6.25 mg/L
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