6,10,14-trimethylpentadecan-2-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38 412, part L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6,10,14-trimethylpentadecan-2-one

EC Number:

207-950-7

EC Name:

6,10,14-trimethylpentadecan-2-one

Cas Number:

502-69-2

Molecular formula:

C18H36O

IUPAC Name:

6,10,14-trimethylpentadecan-2-one

Details on test material:

- Name of test material (as cited in study report): Hexyhycrofarnesylaceton
- Analytical purity: 93.4 %
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added to the test water without any pretreatment

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range: 6.0 - 7.2 cm)
- Weight at study initiation (mean and range, SD): 2.8 g (range: 2.0 - 3.6 g)

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, Ssniff Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: 1 day before and during exposure
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

19 - 20 °C

pH:

7.3 - 7.8

Dissolved oxygen:

8.2 - 9.0 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
5000,
10000,
control

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.8 g/l


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38 412, part 11, preparation from fully demineralised tap water
- Ca/mg ratio: 4:1
- Conductivity: max. 10 micro MHO



OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: LC 50 after 96 h : > 10000 mg/l

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

LC 50 after 48 h: ca. 32 mg/l

Reported statistics and error estimates:

Probit analysis