6,10,14-trimethylpentadecan-2-one

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6,10,14-trimethylpentadecan-2-one

EC Number:

207-950-7

EC Name:

6,10,14-trimethylpentadecan-2-one

Cas Number:

502-69-2

Molecular formula:

C18H36O

IUPAC Name:

6,10,14-trimethylpentadecan-2-one

Details on test material:

- Name of test material (as cited in study report): Hexahydrofarnesylaceton
- Lot/batch No.: 22.04.88

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

Tween 20

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was solved in sterile dilution water using 25 mg/l Tween 20, filtered and subsequently diluted by adding sterile water mixed with 25 mg/l Tween 20. By adding the inoculum and nutrient solution to the dilution the test concentrations were prepared.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 20

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: CHOD

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23 +/- 2 °C

pH:

8.2 - 9.7

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.78,
1.56,
3.13,
12.5,
50.0,
control,
solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml) with stainless steel caps
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2


GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Light intensity and quality: 120 µE/m²s, Osram L 36W/25 universal white



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter

Reference substance (positive control):

yes

Remarks:

Potassiumdichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

4.45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

14.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate