Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 277-492-0 | CAS number: 73507-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity to aquatic invertebrate studies as-
WoE 2. and WoE 3. - GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Remarks:
- WoE 2: yes and WoE 3: not specified
- Vehicle:
- no
- Remarks:
- WoE 2: no and WoE 3: not specified
- Details on test solutions:
- WoE 2: The test solution was prepared by dissolving 50 mg of the test chemical in 100 ml of ADaM’s media achieving a final test concentrations of 500 mg/l. Test chemical concentrations used for the study were 0, 7.5, 15, 30, 60, 120 mg/l, respectively.
WoE 3: no data available - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- WoE 2:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: Eggs of Daphnia magna were obtained from MicroBio tests Kleimoer 15B-9030 MARIAKERKE (GENT) BELGIUM
- Feeding during test: no
- Other: A population of parthenogenetic females of synchronized age structure has been maintained for more than 2 years in the test facility under constant temperature conditions (18 to 22°C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture media (Adams medium') was partly renewed once a week. During the maintenance of test organism, test daphnids were fed with unicellular green algae (Selenestrum capricornutum).
WoE 3:
TEST ORGANISM
- Common name: Water flea - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- WoE 2: 140 mg of CaCO3
WoE 3: no data available - Test temperature:
- WoE 2: 18-22°C
WoE 3: no data available - Nominal and measured concentrations:
- WoE 2: Nominal concentrations: 0, 7.5, 15, 30, 60, 120 mg/l
WoE 3: no data available - Details on test conditions:
- WoE 2:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Light intensity: 1000 – 1500 Lux
WoE 3: no data available - Reference substance (positive control):
- yes
- Remarks:
- WoE 2: potassium dichromate was used as a reference substance for the study and WoE 3: no data available
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE 2
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE 3
- Results with reference substance (positive control):
- WoE 2: The 48 hr EC50 value of reference substance potassium dichromate was determined to be 0.831 mg/l.
WoE 3: no data available - Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism Daphnia magna, the 48hr EC50 value can be expected to be > 50 mg/l. Since the test chemical is readily biodegradable in nature, test chemical was considered as non-toxic and hence, considered to be not classified as per CLP classification criteria.
- Executive summary:
Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test solution was prepared by dissolving 50 mg of the test chemical in 100 ml of ADaM’s media achieving a final test concentrations of 500 mg/l. Test chemical concentrations used for the study were 0, 7.5, 15, 30, 60, 120 mg/l, respectively. Test concentrations were verified analytically by UV-Vis Spectrophotometer. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 18 -22°C, hardness of water 140 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions with light intensity 1000 – 1500 Lux, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. Potassium dichromate was used as a reference substance for the study. The 48 hr EC50 value of reference substance was determined to be 0.831 mg/l. No Immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. As the concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be > 120 mg/L. Thus, based on the EC50 value, chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be 'not classified' as per CLP classification criteria.
Another short term toxicity to aq. Invertebrate study was conducted for 48 hrs for assessing the effect of test chemical. The study was performed in accordance to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system. Daphnia magna was used as a test organism for the study.On the basis of effect on the mobility of the test organism Daphnia magna, the 48 hr EC50 value was determined to be > 50 mg/l. Since the test chemical is readily biodegradable in water, chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be not classified as per the CLP classification criteria.
On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism Daphnia magna, the 48hr EC50 value can be expected to be > 50 mg/l. Since the test chemical is readily biodegradable in nature, test chemical was considered as non-toxic and hence, considered to be not classified as per CLP classification criteria.
Reference
WoE 2:
Table: Assessment of test concentrations
Sr. no. |
Concentrations (mg/l) |
Wavelength (nm) |
Absorbance |
Temperature (°C) |
1 |
blank |
612 |
0.00 |
25 |
2 |
5.00 |
612 |
0.07 |
25 |
3 |
10.00 |
612 |
0.14 |
25 |
4 |
20.00 |
612 |
0.29 |
25 |
5 |
30.00 |
612 |
0.43 |
25 |
6 |
40.00 |
612 |
0.57 |
25 |
7 |
50.00 |
612 |
0.71 |
25 |
8 |
60.00 |
612 |
0.84 |
25 |
9 |
70.00 |
612 |
1.03 |
25 |
10 |
80.00 |
612 |
1.17 |
25 |
11 |
90.00 |
612 |
1.29 |
25 |
12 |
100.00 |
612 |
1.43 |
25 |
13 |
110.00 |
612 |
1.53 |
25 |
14 |
120.00 |
612 |
1.64 |
25 |
The absorbance and concentrations were recorded at 202 nm.
Table: Concentration after analytical Determination
Sr. No |
Concentrations (mg/L) |
Absorbance (mean) (0 hour) |
Analytical Concentrations (0 hour) |
Absorbance (mean) (48 hour) |
Analytical Concentrations (48 hour) |
1 |
blank |
0.00 |
0.00 |
0.00 |
0.00 |
2 |
7.5 |
0.10 |
7.74 |
0.12 |
9.00 |
3 |
15 |
0.22 |
16.26 |
0.25 |
17.70 |
4 |
30 |
0.44 |
31.72 |
0.47 |
33.90 |
5 |
60 |
0.87 |
61.94 |
0.93 |
65.87 |
6 |
120 |
1.56 |
110.63 |
1.61 |
114.60 |
Table: pH, DO AND TEMPERATURE
Test Concentration (mg/L) |
pH |
Dissolved oxygen |
Temperature °C |
||||||
0 Hour |
24 Hour |
48 Hour |
0 Hour |
24 Hour |
48 Hour |
0 Hour |
24 Hour |
48 Hour |
|
Control |
7.9 |
7.9 |
7.6 |
7.8 |
6.9 |
5.2 |
20.0 |
19 |
20 |
7.5 |
7.9 |
7.5 |
7.7 |
7.5 |
6.8 |
5.0 |
20.0 |
19 |
20 |
15 |
7.8 |
7.5 |
7.4 |
7.5 |
6.6 |
5.1 |
20.0 |
19 |
20 |
30 |
7.9 |
7.4 |
7.4 |
7.5 |
6.8 |
4.9 |
20.0 |
19 |
20 |
60 |
7.9 |
7.8 |
7.3 |
7.6 |
6.5 |
5.0 |
20.0 |
19 |
20 |
120 |
7.8 |
7.5 |
7.2 |
7.5 |
6.6 |
4..8 |
20.0 |
19 |
20 |
Table: IMMOBILIZATION AND INHIBITION
Sr. No |
Test Concentrations (mg/L) |
0 Hour |
24 Hour |
48 Hour |
Cumulative Immobilization |
Percent Inhibition |
1 |
Control |
- |
- |
- |
- |
- |
2 |
7.5 |
- |
- |
- |
- |
- |
3 |
15 |
- |
- |
- |
- |
- |
4 |
30 |
- |
- |
- |
- |
- |
5 |
60 |
- |
- |
- |
- |
- |
6 |
120 |
- |
- |
- |
- |
- |
|
|
No abnormality observed |
All daphnids are alive |
No immobility observed |
|
|
WoE 3: no data available
Description of key information
On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism Daphnia magna, the 48hr EC50 value can be expected to be > 50 mg/l. Since the test chemical is readily biodegradable in nature, test chemical was considered as non-toxic and hence, considered to be not classified as per CLP classification criteria.
Key value for chemical safety assessment
Additional information
Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test solution was prepared by dissolving 50 mg of the test chemical in 100 ml of ADaM’s media achieving a final test concentrations of 500 mg/l. Test chemical concentrations used for the study were 0, 7.5, 15, 30, 60, 120 mg/l, respectively. Test concentrations were verified analytically by UV-Vis Spectrophotometer. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 18 -22°C, hardness of water 140 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions with light intensity 1000 – 1500 Lux, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. Potassium dichromate was used as a reference substance for the study. The 48 hr EC50 value of reference substance was determined to be 0.831 mg/l. No Immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. As the concentration of the test chemical being tested has been satisfactorily maintained within ± 20 % of the nominal concentration throughout the test. Therefore, the analysis of the results was based on nominal concentration. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be > 120 mg/L. Thus, based on the EC50 value, chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be 'not classified' as per CLP classification criteria.
Another short term toxicity to aq. Invertebrate study was conducted for 48 hrs for assessing the effect of test chemical. The study was performed in accordance to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system. Daphnia magna was used as a test organism for the study.On the basis of effect on the mobility of the test organism Daphnia magna, the 48 hr EC50 value was determined to be > 50 mg/l. Since the test chemical is readily biodegradable in water, chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be not classified as per the CLP classification criteria.
On the basis of the experimental studies of the read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism Daphnia magna, the 48hr EC50 value can be expected to be > 50 mg/l. Since the test chemical is readily biodegradable in nature, test chemical was considered as non-toxic and hence, considered to be not classified as per CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
