Ammonium phosphinate

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates

On the basis of the experimental studies of the comparatively similar read across chemical and applying the weight of evidence approach, the 48 hr EC50/LC50 value of the test chemical on test organism can be expected to be in the range 433 to > 1200 mg/l.

Toxicity to aquatic algae and cyanobacteria

On the basis of the experimental studies of the comparatively similar read across chemical and applying the weight of evidence approach, the 96 EC50 and 120 hr EC50/LC50/IC50 value of the test chemical on test organism can be expected to be 199.79 mg/l and in the range of 330 to 5080 mg/l, respectively

Additional information

Short term toxicity to aquatic invertebrates

Data available for the comparatively similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

 

Short term toxicity to aquatic invertebrate study was conducted for 48 hrs for assessing the effect of test chemical. The study was performed in accordance with the ISO 6341 15 "Water quality – Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)" in a static system. Daphnia magna was used as a test organism. Test chemical concentrations were not verified analytically. On the basis of effect of test chemical on the mobility of the test organism Daphnia magna, the 48 hr EC0 and EC50 value was determined to be 940 mg/l and > 940 mg/l (nominal concentration).

 

Another short term toxicity to aq. Invertebrate study was conducted for 96 hrs for assessing the effect of test chemical. Study was performed in a static system using Daphnia magna as a test organism. Test organism was originally obtained from Put-In-Bay, Ohio and cultured in the laboratory. Standard reference water (SRF) obtained from University Lake which was then filtered through glass wool was used as a test medium. It is free from organisms and contains all the major ions in concentrations and proportions of a mean surface water of the United States. On the basis of effect on mortality of the test organism Daphnia magna, the 24, 48 and 96 hr LC50 value was determined to be 423, 433 and 292 mg/l.

 

For the test chemical, short term toxicity to Branchiura sowerbyi (Oligochaete) study was conducted for 96 hrs for assessing the effect of test chemical. No analytical monitoring was performed of exposed test chemical concentrations. This test was performed under static conditions at a temperature of 36°C, pH 7.5, dissolved oxygen of 7.5 mg/l, hardness of 220 mg/l as CaCO3 and alkalinity 170 mg/l as CaO3.On the basis of effect on mortality of the test organism Branchiura sowerbyi, the 96 hr LC50 was determined to be 1200 mg/l. It would be generally expected that the LC50 at short time period should be greater than the LC50 obtained in the longer time period. Thus, the 48 LC50 value can be evaluated to be > 1200 mg/l.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 48 hr EC50/LC50 value of the test chemical on test organism can be expected to be in the range 433 to > 1200 mg/l. Based on this value, test chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be not classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Data available for the comparatively similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic algae. The studies are as mentioned below:

 

An acute toxicity test was conducted for 96 hrs for assessing the effect of test chemical on green algae. Microcystis aeruginosa FACHB 315 (green algae) was used as a test organism. The study was performed taking cells from an exponential growth phase culture in a static system at a temperature of 25°C and pH 8.3, respectively. On the basis of effect of test chemical on decrease in growth rate of the test organism, the 96 hr ErC50 value was determined to be 199.79 mg/l (95% C. I. = 153.574 to 259.969 mg/l).

 

Another acute toxicity test was conducted for 120 hrs for assessing the effect of test chemical on green algae. Chlorella vulgaris A-23 (green algae) was used as a test organism. Study was performed in a static system. Modified Prat's mineral medium was used as a test medium. Composition of the medium includes KH2PO4-0.135g, MgSO4.7H2O-0.5g, FeSO4.7H2O-0.003g, Sodium Citrate-0.006g, micronutrients solution-1ml, H2O-1000ml, respectively. The culture was centrifuged, the harvested cells washed with distilled water and resuspended in medium without nitrogen. Test culture was aerated with air containing 3% CO2. 200ml aliquots were distributed into culture vessels containing appropriate amounts of test chemical as a nitrogen source. These test vessels were incubated for 120 hrs (5 days) under test conditions i.e, at a temperature of 26°C, pH 7.0 with light intensity of 6000 lux. After an exposure period of 120 hrs, number of cells, percentage of living cells, average cells size and pH was measured. On the basis of the effect on growth rate and mortality of the test organism, the 120 hr ErC50 and LC50 was determined to be 1300 mg/l and 5080 mg/l.

 

For the test chemical, toxicity to aquatic algae study was conducted for 120 hrs for assessing the effect of test chemical. Chlorella vulgaris(green algae) was used as a test organism. Study was performed in a static system at a temperature of 26°C. On the basis of the effect on growth rate of the test organism, the 120 hr IC50 value was determined to be 330 mg/l.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 96 EC50 and 120 hr EC50/LC50/IC50 value of the test chemical on test organism can be expected to be 199.79 mg/l and in the range of 330 to 5080 mg/l, respectively. Thus, based on these values, test chemical was considered as non-toxic to aquatic algae and hence, considered to be ‘not classified’ as per the CLP classification criteria. 

On the basis of the available information of aquatic toxicity studies, it can be concluded that the test chemical was considered as non-toxicto aquatic organisms at environmental relevant concentrations and hence, considered to be ‘not classified’ as per the CLP classification criteria