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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
experimental data of read across substances
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: as mentiioned below
Principles of method if other than guideline:
WoE report is based on three short term toxicity to aquatic invertebrate studies as-
WoE 2., WoE 3 and WoE 4.
GLP compliance:
not specified
Analytical monitoring:
no
Remarks:
WoE 2 & WoE 4: no and WoE 3: no specified
Vehicle:
not specified
Test organisms (species):
other: WoE 2 & WoE 3: Daphnia magna and WoE 4: Branchiura sowerbyi (other aquatic worms)
Details on test organisms:
WoE 2:
TEST ORGANISM
- Common name: Water flea

WoE 3:
TEST ORGANISM
- Common name: Water flea
- Source: Test organism culture was originally obtained from Put-In-Bay, Ohio.
- Maintenance: Test organism were cultured in the laboratory.

WoE 4:
TEST ORGANISM
- Common name: Oligochaete
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Remarks on exposure duration:
WoE 2: 48 hrs and WoE 3 & WoE 4: 96 hrs
Hardness:
WoE 2 and WoE 3: no data available
WoE 4: 220 mg/l as CaCO3
Test temperature:
WoE 2 and WoE 3: no data available
WoE 4: 36°C
pH:
WoE 2 and WoE 3: no data available
WoE 4: 7.5
Dissolved oxygen:
WoE 2 and WoE 3: no data available
WoE 4: 7.5 mg/l
Details on test conditions:
WoE 2: no data available

WoE 3:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard reference water (SRF) obtained from University Lake which was then filtered through glass wool.
- Other: Standard reference water was described as a laboratory prepared medium which is free from organisms and contains all the major ions in concentrations and proportions of a mean surface water of the United States.

WoE 4:
TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 170 mg/l as CaCO3

Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
940 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: WoE 2
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 940 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: WoE 2
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
423 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: WoE 3
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
433 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: WoE 3
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
292 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: WoE 3
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: WoE 4
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: WoE 4

WoE 2: no data available

WoE 3:

Table: Median tolerance limit of test chemical for Daphnia magna in dilution media.

 

Chemical

Test organism

Medium

TLm (mg/l)

24 hr

48 hr

72 hr

96 hr

Test chemical

Daphnia magna

Standard reference water (SRF)

423*

433*

-

292*

 

WoE 4: no data available

Validity criteria fulfilled:
not specified
Conclusions:
On the basis of the experimental studies of the comparatively similar read across chemical and applying the weight of evidence approach, the 48 hr EC50/LC50 value of the test chemical on test organism can be expected to be in the range 433 to > 1200 mg/l.
Executive summary:

Data available for the comparatively similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

Short term toxicity to aquatic invertebrate study was conducted for 48 hrs for assessing the effect of test chemical. The study was performed in accordance with the ISO 6341 15 "Water quality – Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)" in a static system. Daphnia magna was used as a test organism. Test chemical concentrations were not verified analytically. On the basis of effect of test chemical on the mobility of the test organism Daphnia magna, the 48 hr EC0 and EC50 value was determined to be 940 mg/l and > 940 mg/l (nominal concentration).

Another short term toxicity to aq. Invertebrate study was conducted for 96 hrs for assessing the effect of test chemical. Study was performed in a static system using Daphnia magna as a test organism. Test organism was originally obtained from Put-In-Bay, Ohio and cultured in the laboratory. Standard reference water (SRF) obtained from University Lake which was then filtered through glass wool was used as a test medium. It is free from organisms and contains all the major ions in concentrations and proportions of a mean surface water of the United States. On the basis of effect on mortality of the test organism Daphnia magna, the 24, 48 and 96 hr LC50 value was determined to be 423, 433 and 292 mg/l.

For the test chemical, short term toxicity to Branchiura sowerbyi (Oligochaete) study was conducted for 96 hrs for assessing the effect of test chemical. No analytical monitoring was performed of exposed test chemical concentrations. This test was performed under static conditions at a temperature of 36°C, pH 7.5, dissolved oxygen of 7.5 mg/l, hardness of 220 mg/l as CaCO3 and alkalinity 170 mg/l as CaO3.On the basis of effect on mortality of the test organism Branchiura sowerbyi, the 96 hr LC50 was determined to be 1200 mg/l. It would be generally expected that the LC50 at short time period should be greater than the LC50 obtained in the longer time period. Thus, the 48 LC50 value can be evaluated to be > 1200 mg/l.

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 48 hr EC50/LC50 value of the test chemical on test organism can be expected to be in the range 433 to > 1200 mg/l. Based on this value, test chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be not classified as per the CLP classification criteria.

Description of key information

On the basis of the experimental studies of the comparatively similar read across chemical and applying the weight of evidence approach, the 48 hr EC50/LC50 value of the test chemical on test organism can be expected to be in the range 433 to > 1200 mg/l.

Key value for chemical safety assessment

Additional information

Data available for the comparatively similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

 

Short term toxicity to aquatic invertebrate study was conducted for 48 hrs for assessing the effect of test chemical. The study was performed in accordance with the ISO 6341 15 "Water quality – Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)" in a static system. Daphnia magna was used as a test organism. Test chemical concentrations were not verified analytically. On the basis of effect of test chemical on the mobility of the test organism Daphnia magna, the 48 hr EC0 and EC50 value was determined to be 940 mg/l and > 940 mg/l (nominal concentration).

 

Another short term toxicity to aq. Invertebrate study was conducted for 96 hrs for assessing the effect of test chemical. Study was performed in a static system using Daphnia magna as a test organism. Test organism was originally obtained from Put-In-Bay, Ohio and cultured in the laboratory. Standard reference water (SRF) obtained from University Lake which was then filtered through glass wool was used as a test medium. It is free from organisms and contains all the major ions in concentrations and proportions of a mean surface water of the United States. On the basis of effect on mortality of the test organism Daphnia magna, the 24, 48 and 96 hr LC50 value was determined to be 423, 433 and 292 mg/l.

 

For the test chemical, short term toxicity to Branchiura sowerbyi (Oligochaete) study was conducted for 96 hrs for assessing the effect of test chemical. No analytical monitoring was performed of exposed test chemical concentrations. This test was performed under static conditions at a temperature of 36°C, pH 7.5, dissolved oxygen of 7.5 mg/l, hardness of 220 mg/l as CaCO3 and alkalinity 170 mg/l as CaO3.On the basis of effect on mortality of the test organism Branchiura sowerbyi, the 96 hr LC50 was determined to be 1200 mg/l. It would be generally expected that the LC50 at short time period should be greater than the LC50 obtained in the longer time period. Thus, the 48 LC50 value can be evaluated to be > 1200 mg/l.

 

On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the 48 hr EC50/LC50 value of the test chemical on test organism can be expected to be in the range 433 to > 1200 mg/l. Based on this value, test chemical was considered as non-toxic to aquatic invertebrates and hence, considered to be not classified as per the CLP classification criteria.