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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data from various test chemicals
Justification for type of information:
Data for the target chemical is summarized bases on experimental data from various test chemicals
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
read-across source
Reference
Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from publication
Qualifier:
according to guideline
Guideline:
other: Standard irritation protocol
Principles of method if other than guideline:
Primary Skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition.
GLP compliance:
not specified
Species:
rabbit
Strain:
not specified
Details on test animals or test system and environmental conditions:
No Data Available
Type of coverage:
occlusive
Preparation of test site:
clipped
Remarks:
Intact or abraded skin
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
500 mg (0.5ml)
Duration of treatment / exposure:
4 hours
Observation period:
24/48/72 hours
Number of animals:
6 rabbits
Details on study design:
TEST SITE
- Area of exposure: clipped hair free back of six rabbis
- % coverage: one-inch square/ site

- Type of wrap if used: Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
- Time after start of exposure: No data

SCORING SYSTEM: The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved.
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 4 h
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Mild skin irritation was present on the treated area after 4 hours exposure period
Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period.
Executive summary:

Primary skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition. Briefly, the backs of six rabbits were clipped free of hair. Each test material was placed on two one-inch square sites on the same animal. One site was intact and the other site was abraded. Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed. The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved. Mild skin irritation was observed on treated area after 4 hours exposure period. The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period and hence it is likely to classify as a skin irritant.

Reason / purpose for cross-reference:
read-across source
Reference
Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from publication
Qualifier:
according to guideline
Guideline:
other: Standard skin irritation protocols
Principles of method if other than guideline:
Primary Skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition.
GLP compliance:
not specified
Species:
rabbit
Strain:
not specified
Details on test animals or test system and environmental conditions:
No Data Available
Type of coverage:
occlusive
Preparation of test site:
clipped
Remarks:
Intact or abraded skin
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
500 mg (0.5ml)
Duration of treatment / exposure:
4 hours
Observation period:
24/48/72 hours
Number of animals:
6 rabbits
Details on study design:
TEST SITE
- Area of exposure: clipped hair free back of six rabbis
- % coverage: one-inch square/ site

- Type of wrap if used: Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
- Time after start of exposure: No data

SCORING SYSTEM: The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved.
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 4 h
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Mild skin irritation was present in treated area after 4 hours exposure period
Other effects:
No data
Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period.
Executive summary:

Primary skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition. Briefly, the backs of six rabbits were clipped free of hair. Each test material was placed on two one-inch square sites on the same animal. One site was intact and the other site was abraded. Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed. The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved. Mild skin irritation was observed on treated area after 4 hours exposure period. The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period and hence it is likely to classify as a skin irritant.

Data source

Reference
Reference Type:
publication
Title:
WoE of skin irritation study for CAS no 7803-65-8
Author:
Sustainability Support Services (Europe) AB
Year:
2019
Bibliographic source:
WoE report, Sustainability Support Services (Europe) AB, 2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Standard skin irritation protocols
Principles of method if other than guideline:
WoE for the target CAS is summarized based on data from various test chemicals
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium phosphinate
EC Number:
232-266-0
EC Name:
Ammonium phosphinate
Cas Number:
7803-65-8
Molecular formula:
H3N.H3O2P
IUPAC Name:
ammonium phosphinate
Details on test material:
- Name of test material : Ammonium phosphinate
- Molecular formula : H4NO2P
- Molecular weight : 83.0264 g/mol
- Smiles notation : [NH4+].[O-]P=O
- InChl : 1S/H3N.H3O2P/c;1-3-2/h1H3;3H2,(H,1,2)
- Substance type: Inorganic
- Physical state: Solid

Test animals

Species:
rabbit
Strain:
not specified
Details on test animals or test system and environmental conditions:
No Data Available

Test system

Type of coverage:
occlusive
Preparation of test site:
clipped
Remarks:
Intact or abraded skin
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
500 mg (0.5ml)
Duration of treatment / exposure:
4 hours
Observation period:
24/48/72 hours
Number of animals:
6 rabbits
Details on study design:
TEST SITE
- Area of exposure: clipped hair free back of six rabbis
- % coverage: one-inch square/ site

- Type of wrap if used: Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
- Time after start of exposure: No data

SCORING SYSTEM: The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved.

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 4 h
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Mild skin irritation was present on the treated area after 4 hours exposure period
Other effects:
No data

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
The test chemical was considered to be irritating to the skin of rabbits after 4 hours exposure period.
Executive summary:

Data available for the various test chemicals was reviewed to determine the toxic nature of the test chemical. The studies are as mentioned below:

Primary skin irritation study of the two test chemicals was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition. Briefly, the backs of six rabbits were clipped free of hair. Each test material was placed on two one-inch square sites on the same animal. One site was intact and the other site was abraded. Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed. The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved. Mild skin irritation was observed on treated area after 4 hours exposure period. The test chemicals was considered to be irritating to the skin of rabbits after 4 hours exposure period and hence it is likely to classify as a skin irritant.

Based on the data available and applying the weight of evidence approach, the test chemical was considered to be irritating to the skin of rabbits after 4 hours exposure period.