Ammonium phosphinate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data from various test chemicals

Justification for type of information:

Data for the target chemical is summarized bases on experimental data from various test chemicals

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

read-across source

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from publication

Qualifier:

according to guideline

Guideline:

other: Standard irritation protocol

Principles of method if other than guideline:

Primary Skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition.

GLP compliance:

not specified

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

No Data Available

Type of coverage:

occlusive

Preparation of test site:

clipped

Remarks:

Intact or abraded skin

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

500 mg (0.5ml)

Duration of treatment / exposure:

4 hours

Observation period:

24/48/72 hours

Number of animals:

6 rabbits

Details on study design:

TEST SITE
- Area of exposure: clipped hair free back of six rabbis
- % coverage: one-inch square/ site

- Type of wrap if used: Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
- Time after start of exposure: No data

SCORING SYSTEM: The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved.

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 4 h

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Mild skin irritation was present on the treated area after 4 hours exposure period

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period.

Executive summary:

Primary skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition. Briefly, the backs of six rabbits were clipped free of hair. Each test material was placed on two one-inch square sites on the same animal. One site was intact and the other site was abraded. Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed. The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved. Mild skin irritation was observed on treated area after 4 hours exposure period. The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period and hence it is likely to classify as a skin irritant.

Cross-reference 2

Reason / purpose for cross-reference:

read-across source

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from publication

Qualifier:

according to guideline

Guideline:

other: Standard skin irritation protocols

Principles of method if other than guideline:

Primary Skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition.

GLP compliance:

not specified

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

No Data Available

Type of coverage:

occlusive

Preparation of test site:

clipped

Remarks:

Intact or abraded skin

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

500 mg (0.5ml)

Duration of treatment / exposure:

4 hours

Observation period:

24/48/72 hours

Number of animals:

6 rabbits

Details on study design:

TEST SITE
- Area of exposure: clipped hair free back of six rabbis
- % coverage: one-inch square/ site

- Type of wrap if used: Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
- Time after start of exposure: No data

SCORING SYSTEM: The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved.

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 4 h

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Mild skin irritation was present in treated area after 4 hours exposure period

Other effects:

No data

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period.

Executive summary:

Primary skin irritation study of test chemical was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition. Briefly, the backs of six rabbits were clipped free of hair. Each test material was placed on two one-inch square sites on the same animal. One site was intact and the other site was abraded. Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed. The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved. Mild skin irritation was observed on treated area after 4 hours exposure period. The test material was considered to be irritating to the skin of rabbits after 4 hours exposure period and hence it is likely to classify as a skin irritant.

Data source

Reference

Reference Type:

publication

Title:

WoE of skin irritation study for CAS no 7803-65-8

Author:

Sustainability Support Services (Europe) AB

Year:

2019

Bibliographic source:

WoE report, Sustainability Support Services (Europe) AB, 2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Standard skin irritation protocols

Principles of method if other than guideline:

WoE for the target CAS is summarized based on data from various test chemicals

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium phosphinate

EC Number:

232-266-0

EC Name:

Ammonium phosphinate

Cas Number:

7803-65-8

Molecular formula:

H3N.H3O2P

IUPAC Name:

ammonium phosphinate

Details on test material:

- Name of test material : Ammonium phosphinate
- Molecular formula : H4NO2P
- Molecular weight : 83.0264 g/mol
- Smiles notation : [NH4+].[O-]P=O
- InChl : 1S/H3N.H3O2P/c;1-3-2/h1H3;3H2,(H,1,2)
- Substance type: Inorganic
- Physical state: Solid

Test animals

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

No Data Available

Test system

Type of coverage:

occlusive

Preparation of test site:

clipped

Remarks:

Intact or abraded skin

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

500 mg (0.5ml)

Duration of treatment / exposure:

4 hours

Observation period:

24/48/72 hours

Number of animals:

6 rabbits

Details on study design:

TEST SITE
- Area of exposure: clipped hair free back of six rabbis
- % coverage: one-inch square/ site

- Type of wrap if used: Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No
- Time after start of exposure: No data

SCORING SYSTEM: The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved.

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 4 h

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Mild skin irritation was present on the treated area after 4 hours exposure period

Other effects:

No data

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The test chemical was considered to be irritating to the skin of rabbits after 4 hours exposure period.

Executive summary:

Data available for the various test chemicals was reviewed to determine the toxic nature of the test chemical. The studies are as mentioned below:

Primary skin irritation study of the two test chemicals was conducted on the intact or abraded skin of six rabbits to observe its irritation efficacy under occlusive condition. Briefly, the backs of six rabbits were clipped free of hair. Each test material was placed on two one-inch square sites on the same animal. One site was intact and the other site was abraded. Each test site was covered with cotton gauze pads and secured with tape in contact with the skin for 24 h. The entire trunk of the animal was wrapped with rubberized cloth or other occlusive impervious material to retard evaporation of the test substance. After the 24-h exposure period, the wrappings were removed. The sites were scored after approximately 1 h for erythema and edema, using a standard Draize scoring system on a scale of 0–4 for each parameter (maximum of 8.0 Primary Irritation Index). Scoring was repeated at 24, 48 and 72 h post-exposure or until all irritation resolved. Mild skin irritation was observed on treated area after 4 hours exposure period. The test chemicals was considered to be irritating to the skin of rabbits after 4 hours exposure period and hence it is likely to classify as a skin irritant.

Based on the data available and applying the weight of evidence approach, the test chemical was considered to be irritating to the skin of rabbits after 4 hours exposure period.